1887

Abstract

Abstract

Other workers have found that clinical isolates of exhibit very extensive DNA sequence polymorphisms when they are examined by ribotyping or some other genomic sequence characterization technique. In fact, it is rare to find similar clones, much less identical ones, among isolates. We found that the levels of divergence between the 16S ribosomal DNA sequences of individual organisms and the consensus sequence of the five isolates which we examined ranged from 0.2 to 0.5%. In contrast, other workers have shown that levels of divergence between the 16S ribosomal DNA sequence of and the 16S ribosomal DNA sequences of four other species range from 2.7 to 8.0%. Our results show that the 16S ribosomal DNA is not very polymorphic and support the conclusion that is a unique species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-44-2-320
1994-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/44/2/ijs-44-2-320.html?itemId=/content/journal/ijsem/10.1099/00207713-44-2-320&mimeType=html&fmt=ahah

References

  1. Akopyanz N., Bukanov N. O., Westblom T. U., Kresovich S., Berg D. E. 1992; DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res. 20 5137 5142
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc. Natl. Acad. Sci. USA 75 4801 4805
    [Google Scholar]
  3. Clayton C. L., Kleanthous H., Morgan D. D., Puckey L., Tabaqchali S. 1993; Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and restriction fragment length polymorphism analysis. J. Clin. Microbiol. 31 1420 1425
    [Google Scholar]
  4. Dewhirst F. E. Personal communication
    [Google Scholar]
  5. Dewhirst F. E., Eaton K. A., Paster S. Personal communication
    [Google Scholar]
  6. Eaton K. A., Dewhirst F. E., Radin M. J., Fox J. G., Paster B. J., Krakowka S., Morgan D. R. 1993; Helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. Int. J. Syst. Bacteriol. 43 99 106
    [Google Scholar]
  7. Edwards U., Rogall T., Blocker H., Emde M., Böttger E. C. 1989; Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16Sʹribosomal RNA. Nucleic Acids Res. 17 7843 7853
    [Google Scholar]
  8. Höök-Nikanne J., Solin M.-L., Kosunen T. U., Kaartinen M. 1991; Comparison of partial 16S rRNA sequences of different Helicobacter pylori strains, Helicobacter mustelae and a gastric campylobacter-like organism (GCLO). Syst. Appl. Microbiol. 14 270 274
    [Google Scholar]
  9. Linton D., Moreno M., Owen R. J., Stanley J. 1992; 16S rrn gene copy number in Helicobacter pylori and its application to molecular typing. J. Appl. Bacteriol. 73 501 506
    [Google Scholar]
  10. Moore R. A., Kureishi A., Wong S., Bryan L. E. 1993; Categorization of clinical isolates of Helicobacter pylori on the basis of restriction digest analyses of polymerase chain reaction-amplified ureC genes. J. Clin. Microbiol. 31 1334 1335
    [Google Scholar]
  11. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual , 2nd ed.. p. Append E.3 Cold Spring Harbor Laboratory Press, Cold Spring Harbor; N.Y.:
    [Google Scholar]
  12. Taylor D. E., Eaton M., Chang N., Salama S. M. 1992; Construction of Helicobacter pylori genome map and demonstration of diversity at the genome level. J. Bacteriol. 174 6800 6806
    [Google Scholar]
  13. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev. 51 221 271
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-44-2-320
Loading
/content/journal/ijsem/10.1099/00207713-44-2-320
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error