1887

Abstract

An anaerobic and thermophilic alkaliphile, strain JW/YL23-2 (T = type strain), was isolated from sewage sludge obtained from a sewage plant in Atlanta, Ga. At pH 10.1 and 50°C, the doubling time of this strain was 19 min. Strain JW/YL23-2, a motile rod-shaped bacterium with 2 to 12 peritrichous flagella, exhibited a negative Gram stain reaction but was gram-type positive as judged by the polymyxin B test. No heat-stable (85°C, 15 min) endospores were detected. At 50°C, growth occurred at pH values ranging from 7.0 to 11.0; the optimum pH was 9.6 to 10.1. The temperature range for growth ranged from 27 to 57.5°C; the optimum temperature was 48 to 51°C (pH 10.1). Dissimilatory sulfate reduction was not detected. The organism utilized glucose, fructose, sucrose, maltose, cellobiose, and Casamino Acids. The DNA G+C content was 32 mol% (as determined by chemical analysis). A 16S rRNA sequence analysis revealed a 2% inferred evolutionary distance to . However, the cell wall type of strain JW/YL23-2 was A4β (L-Orn-D-Asp), while that of was A1τ(-diaminopimelic acid direct). On the basis of the alkaline pH values and high temperatures for optimal growth, the inability to form spores, and other characteristics different from characteristics, strain JW/YL-23-2 was placed in a new species, ; JW/YL23-2 (= DSM 7309) is the type strain of this new species.

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/content/journal/ijsem/10.1099/00207713-44-1-111
1994-01-01
2020-01-19
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http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-44-1-111
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