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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 44, Issue 1

sp. nov., an Anaerobic and Thermotolerant Facultative Alkaliphile Free

Abstract

An anaerobic and thermophilic alkaliphile, strain JW/YL23-2 (T = type strain), was isolated from sewage sludge obtained from a sewage plant in Atlanta, Ga. At pH 10.1 and 50°C, the doubling time of this strain was 19 min. Strain JW/YL23-2, a motile rod-shaped bacterium with 2 to 12 peritrichous flagella, exhibited a negative Gram stain reaction but was gram-type positive as judged by the polymyxin B test. No heat-stable (85°C, 15 min) endospores were detected. At 50°C, growth occurred at pH values ranging from 7.0 to 11.0; the optimum pH was 9.6 to 10.1. The temperature range for growth ranged from 27 to 57.5°C; the optimum temperature was 48 to 51°C (pH 10.1). Dissimilatory sulfate reduction was not detected. The organism utilized glucose, fructose, sucrose, maltose, cellobiose, and Casamino Acids. The DNA G+C content was 32 mol% (as determined by chemical analysis). A16S rRNA sequence analysis revealed a 2% inferred evolutionary distance to . However, the cell wall type of strain JW/YL23-2 was A4β (-Orn--Asp), while that of was (-diaminopimelic acid direct). On the basis of the alkaline pH values and high temperatures for optimal growth, the inability to form spores, and other characteristics different from C. characteristics, strain JW/YL-23-2 was placed in a new species, ; JW/YL23-2 (= DSM 7309) is the type strain of this new species.

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Copyright 1994, International Union of Microbiological Societies
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1994-01-01
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References

  1. Ausubel F. M.et al. 1989 Current protocols in molecular biology 2.4.1 2.4.5 Wiley Interscience; New York:
    [Google Scholar]
  2. Beuscher N., Mayer F., Gottschalk G. 1974; Citrate lyase from Rhodopseudomonas gelatinosa: purification, electron microscopy and subunit structure. Arch. Microbiol. 100 307 328
    [Google Scholar]
  3. Biggin M. D., Gibson T. J., Hing G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Natl. Acad. Sci. USA 80 3963 3965
    [Google Scholar]
  4. Blotevogel K., Fischer U., Mocha M., Jannsen S. 1985; Methanobacterium thermoalcaliphilum spec, nov., a new moderately alkaliphilic and thermophilic autotrophic methanogen. Arch. Microbiol. 142 211 217
    [Google Scholar]
  5. Boone D. R., Worakit S., Mathrani I. M., Mah R. A. 1986; Alkaliphilic methanogens from high-pH lake sediments. Syst. Appl. Microbiol. 7 230 234
    [Google Scholar]
  6. De Soete G. 1983; A least square algorithm for fitting additive trees to proximity data. Psychometrika 48 621 626
    [Google Scholar]
  7. Frasca J. M., Parks V. R. 1965; A routine technique for double-staining ultrathin sections using uranyl and lead salts. J. Cell Biol. 25 157 161
    [Google Scholar]
  8. Grant W. D., Horikoshi K. 1988; Alkaliphiles. FEMS Symp. 49 346 366
    [Google Scholar]
  9. Grant W. D., Mwatha W. E., Jones B. E. 1990; Alkaliphiles: ecology, diversity and applications. FEMS Microbiol. Rev. 75 255 270
    [Google Scholar]
  10. Horikoshi K. 1990 Microorganisms in alkaline environments Kodansha; Tokyo:
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. 21 132 Munro H. N. Mammalian protein metabolism Academic Press; New York:
    [Google Scholar]
  12. Kellenberger E., Ryter A., Sechaud J. 1958; Electron microscope study of DNA-containing plasma. II. Vegetative and mature phage DNA as compared with normal bacterial nucleosides in different physiological states. J. Biophys. Biochem. Cytol. 4 671 678
    [Google Scholar]
  13. Krulwich T. A., Guffanti A. A. 1989; Alkalophilic bacteria. Annu. Rev. Microbiol. 43 435 463
    [Google Scholar]
  14. Lane D. J., Pace B., Olsen G. J., Stahl D. A. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. USA 82 6955 6959
    [Google Scholar]
  15. Li Y., Mandelco L., Wiegel J. 1993; Isolation and characterization of a moderately thermophilic alkaliphile, Clostridium paradoxum sp. nov. Int. J. Syst. Bacteriol. 43 450 460
    [Google Scholar]
  16. Liu Y., Boone D. R., Choy C. 1990; Methanohalophilus oregonense sp. nov., a methylotrophic methanogen from an alkaline, saline aquifer. Int. J. Syst. Bacteriol. 40 111 116
    [Google Scholar]
  17. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3 208 218
    [Google Scholar]
  18. Mathrani I. M., Boone D. R., Mah R. A., Fox G. E., Lau P. P. 1988; Methanohalophilus zhilinae sp. nov., an alkaliphilic, halophilic, methylotrophic methanogen. Int. J. Syst. Bacteriol. 38 139 142
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int. J. Syst. Bacteriol. 39 159 167
    [Google Scholar]
  20. Olsen G. J., Overbeek R., Larsen N., Marsh T. L., McCaughey M. J., Maciukenas M. A., Kuan W. M., Macke T. J., Woese C. R. 1992; The ribosomal database project. Nucleic Acids Res.. 20 (Suppl.) 2199 2200
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74 5463 5467
    [Google Scholar]
  22. Schleifer K. H., Kandier O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36 407 477
    [Google Scholar]
  23. Shiba H., Yamamoto H., Horikoshi K. 1989; Isolation of strictly anaerobic halophiles from the aerobic surface sediments of hypersaline environments in California and Nevada. FEMS Microbiol. Lett. 57 191 196
    [Google Scholar]
  24. Souza K. A., Deal P. H., Mack H. M., Turnbill C. E. 1974; Growth and reproduction of microorganisms under extremely alkaline conditions. Appl. Microbiol. 28 1066 1068
    [Google Scholar]
  25. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26 31 43
    [Google Scholar]
  26. Valentine R. C., Shapiro B. M., Stadtman E. R. 1968; Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from E. coli. Biochemistry 7 2143 2152
    [Google Scholar]
  27. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol. 171 6455 6467
    [Google Scholar]
  28. Whitman W. B., Sohn S., Caras D. S., Premanchandran U. 1986; Isolation and characterization of 22 mesophilic methanococci. Syst. Appl. Microbiol. 7 235 240
    [Google Scholar]
  29. Wiegel J. 1981; Distinction between the gram reaction and the Gram type of bacteria. Int. J. Syst. Bacteriol. 31 88
    [Google Scholar]
  30. Wiegel J. 1992; The obligate anaerobic thermophilic bacteria. 105 184 Kristjannson J. K. Thermophilic bacteria CRC Press; Boca Raton, Fla:
    [Google Scholar]
  31. Wiegel J., Quandt L. 1982; Determination of the Gram type using the reaction between polymyxim B and lipopolysac- charides of the outer cell wall of whole bacteria. J. Gen. Microbiol. 128 2261 2270
    [Google Scholar]
  32. Woese C. R., Gutell R., Gupta R., Noller H. F. 1983; Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol. Rev. 47 621 669
    [Google Scholar]
  33. Woese C. R., Sogin M., Stahl D. A., Lewis B. J., Bonen L. 1976; A comparison of the 16S ribosomal RNAs from mesophilic and thermophilic bacilli. J. Mol. Evol. 7 197 213
    [Google Scholar]
  34. Worakit S., Boone D. R., Mah R. A., Abdel-Samie M., El-Haiwagi M. M. 1986; Methanobacterium alcaliphilum sp. nov., an H2-utilizing methanogen that grows at high pH values. Int. J. Syst. Bacteriol. 36 380 382
    [Google Scholar]
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Clostridium thermoalcaliphilum sp. nov., an Anaerobic and Thermotolerant Facultative Alkaliphile
Int J Syst Evol Microbiol 44, 111 (1994); https://doi.org/10.1099/00207713-44-1-111
/content/journal/ijsem/10.1099/00207713-44-1-111
/content/journal/ijsem/10.1099/00207713-44-1-111
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