The base compositions of DNAs from nine strains, as determined by high-performance liquid chromatography, were 41 to 42 mol% G+C. Chromosomal DNAs derived from these strains were found to be extremely resistant to many restriction endonucleases, and a restriction analysis revealed the presence of a -like methylase or both - and -like methylases in all of the strains examined. Genomic Southern hybridization in which a synthetic oligonucleotide probe ( probe) was used showed that members of the genus might have multiple homologs, and the hybridization signal patterns observed with the DNAs of strains were different from each other.


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