1887

Abstract

The 16S ribosomal DNA sequence of was determined and compared with the corresponding gene sequences of and has been reported to exhibit little chromosomal DNA homology with the other chlamydial species, and its phylogenetic relationships within the genus have not been described. A polymerase chain reaction was employed to determine the 16S rRNA gene sequence of . Ten primers from the sequences were used to amplify a template in overlapping segments of the gene. Sequence data for 1,554 bases indicated that the levels of homology of with and were 96.19 and 94.07%, respectively. These data support the results of previous biochemical and developmental studies indicating that is more closely related to than to

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-43-3-610
1993-07-01
2022-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/43/3/ijs-43-3-610.html?itemId=/content/journal/ijsem/10.1099/00207713-43-3-610&mimeType=html&fmt=ahah

References

  1. Campbell J. F., Barnes R. C., Kozarsky P. E., Spike J. A. 1991; Culture-confirmed pneumonia due to Chlamydia pneumoniae. J. Infect. Dis 164:411–13
    [Google Scholar]
  2. Campbell L. A., Kuo C. C., Grayston J. T. 1987; Characterization of the new chlamydia agent, TWAR, as a unique organism by restriction endonuclease analysis and DNA-DNA hybridization. J. Clin. Microbiol 25:1911–1916
    [Google Scholar]
  3. Campbell L. A., Kuo C. C., Grayston J. T. 1990; Structural and antigenic analysis of Chlamydia pneumoniae. Infect. Immun 58:93–97
    [Google Scholar]
  4. Chen K., Neimark H., Rumore P., Steinman C. R. 1989; Broad range DNA probes for detecting and amplifying eubac-terial nucleic acids. FEMS Microbiol. Lett 48:19–24
    [Google Scholar]
  5. Chirgwin K., Roblin P. M., Gelling M., Hammerschlag M. R., Schachter J. 1991; Infection with Chlamydia pneumoniae in Brooklyn. J. Infect. Dis 163:757–761
    [Google Scholar]
  6. Gaydos C. A., Quinn T. C., Eiden J. J. 1992; Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene. J. Clin. Microbiol 30:796–800
    [Google Scholar]
  7. Grayston J. T., Campbell L. A., Kuo C. C., Mordhurst C. H., Saikku P., Thom D. H., Wang S. P. 1990; A new respiratory tract pathogen: Chlamydia pneumoniae, strain TWAR. J. Infect. Dis 161:618–625
    [Google Scholar]
  8. Grayston J. T., Kuo C. C., Campbell L. A., Wang S. P. 1989; Chlamydia pneumoniae sp. nov. for Chlamydia sp. strain TWAR. Int. J. Syst. Bacteriol 39:88–90
    [Google Scholar]
  9. Grayston J. T., Kuo C. C., Wang S. P., Altman J. 1986; A new Chlamydia psittaci strain, TWAR, isolated from acute respiratory tract infections. N. Engl. J. Med 315:161–168
    [Google Scholar]
  10. Kuo C. C., Chen H. H., Wang S. P., Grayston J. T. 1986; Identification of a new group of Chlamydia psittaci strains called TWAR. J. Clin. Microbiol 24:1034–1037
    [Google Scholar]
  11. Kuo C. C., Chi E. Y., Grayston J. T. 1988; Ultrastructure study of entry of Chlamydia strain TWAR into HeLa cells. Infect. Immun 56:1668–1672
    [Google Scholar]
  12. Lane D. J., Pace B., Olsten G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci USA: 826955–6959
    [Google Scholar]
  13. Marrie T. J., Grayston J. T., Wang S. P., Kuo C. C. 1987; Pneumonia associated with the TWAR strain of chlamydia. Ann. Intern. Med 106:507–511
    [Google Scholar]
  14. Moulder J. W. 1988; Characteristics of chlamydiae. p. 3–19 In Barron A. L. (ed.) Microbiology of Chlamydia CRC Press, Inc.; Boca Raton, Fla:
    [Google Scholar]
  15. Reiman D. A., Loutit J. S., Schmidt T. M., Falkow S., Tompkins L. C. 1990; The agent of bacillary angiomatosis: an approach to the identification of uncultured pathogens. N. Engl. J. Med 323:1573–1580
    [Google Scholar]
  16. Reiman D. A., Schmidt T. M., MacDermott R. P., Falkow S. 1992; Identification of the uncultured bacillus of Whip-pie’s disease. N. Engl. J. Med 327:293–301
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor; N.Y:
    [Google Scholar]
  18. Sanger F., Nicklen S., Couison A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci USA: 74:5463–5467
    [Google Scholar]
  19. Ward M. E. 1988; The chlamydial developmental cycle. p. 71–95 In Barron A. L. (ed.) Microbiology of Chlamydia CRC Press, Inc.; Boca Raton, Fla:
    [Google Scholar]
  20. Weisburg W. G., Hatch T. P., Woese C. R. 1986; Eubac terial origin of chlamydiae. J. Bacteriol 167:570–574
    [Google Scholar]
  21. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev 51:221–271
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-43-3-610
Loading
/content/journal/ijsem/10.1099/00207713-43-3-610
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error