1887

Abstract

Quin's oval is a relatively large bacterium often seen in the rumens of sheep fed diets containing some readily fermented carbohydrates. It has not been obtained in axenic cultures, but a number of its features have been determined by various methods, such as studying cell suspensions purified from rumen fluid by differential centrifugation. We obtained similarly purified suspensions from a sheep fed a diet containing a large amount of molasses. Nearly complete 16S rRNA sequence analysis of these cells as well as cells of subsp. GA192 (ATCC 12561; type strain) and subsp. HD (ATCC 27209) was done. These sequences were compared with those of other bacteria. Evolutionary distance estimates indicated that Quin's oval was most closely related to the group in the gram-positive phylum but that it belongs in a new genus. We propose the name gen. nov., sp. nov., with its description based on previously known features.

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1993-04-01
2022-08-08
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References

  1. Balch W. E., Wolfe R. S. 1976; New approach to the cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid (HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressurized atmosphere. Appl. Environ. Microbiol. 32:781–791
    [Google Scholar]
  2. Brough B. E., Reid T. C., Howard B. H. 1970; The biochemistry of the rumen bacterium “Quin’s oval.” Part 1. Fermentation of carbohydrates. N. Z. J. Sci. 13:570–575
    [Google Scholar]
  3. Bryant M. P. 1972; Commentary on the Hungate technique for culture of anaerobic bacteria. Am. J. Clin. Nutr. 25:1324–1328
    [Google Scholar]
  4. Bryant M. P. 1984; Genus IX. Selenomonas Von Prowazek. 650–653 In Kreig N. R. (ed.) Bergey’s manual of systematic bacteriology vol. 1 Williams and Wilkins; Baltimore:
    [Google Scholar]
  5. De Soete G. 1973; A least-squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626
    [Google Scholar]
  6. Every D. D., Howard B. H. 1970; The biochemistry of the rumen bacterium “Quin’s oval.” Part 3. The storage polysaccharide. N. Z. J. Sci. 13:584–590
    [Google Scholar]
  7. Green C. J., Stewart G. C., Hollis M. A., Void B. S., Bott K. F. 1985; Nucleotide sequence of Bacillus subtilis ribosomal RNA Operon, rrnB. Gene 37:261–266
    [Google Scholar]
  8. Hungate R. E. 1950; The anaerobic mesophilic cellulolytic bacteria. Bacteriol. Rev. 14:1–19
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. 21–132 In Munro H. N. (ed.) Mammalian protein metabolism Academic Press, Inc.; New York:
    [Google Scholar]
  10. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysis. Proc. Natl. Acad. Sci. USA 82:6955–6959
    [Google Scholar]
  11. McGaughey C. A., Sellers K. C. 1948; Microflora of the rumen. Nature (London) 161:1014–1015
    [Google Scholar]
  12. Olsen G. J., Lane D. J., Grovannoni S. J., Pace N. R., Stahl D. A. 1986; Microbial ecology and evolution. A ribosomal RNA approach. 337–365 In Ornston L. N., Balows A., Bauman P. (ed.) Annual reviews of microbiology vol. 40 Annual Reviews, Inc.; Palo Alto, Calif:
    [Google Scholar]
  13. Orpin C. G. 1972; The culture in vitro of the rumen bacterium Quin’s oval. J. Gen. Microbiol. 73:523–530
    [Google Scholar]
  14. Orpin C. G. 1976; The characterization of the rumen bacterium Eadies’ oval, Magnoovum gen. nov. eadii sp. nov. Arch. Microbiol. 111:155–159
    [Google Scholar]
  15. Prins R. A. 1971; Isolation, culture and fermentation characteristics of Selenomonas ruminantium var. bryanti. J. Bacteriol. 105:820–825
    [Google Scholar]
  16. Quin J. I. 1943; Studies on the alimentary tracts of merino sheep in south Africa. VII. Fermentation in the forestomachs of sheep. Onderstepoort J. Vet. Sci. Anim. Ind. 18:91–112
    [Google Scholar]
  17. Scheifinger C. C, Latham M. J., Wolin M. J. 1975; Relationship of lactate dehydrogenase specificity and growth rate to lactate metabolism by Selenomonas ruminantium. Appl. Microbiol. 30:916–921
    [Google Scholar]
  18. Stackebrandt E., Pohla H., Kroppenstedt R., Hippe H., Woese C. R. 1985; 16S rRNA analysis of Sporomusa, Selenomonas, and Megasphaera: on the phylogenetic origin of gram-positive eubacteria. Arch. Microbiol. 143:270–276
    [Google Scholar]
  19. Stahl D. A., Flesher B., Mansfield H. R., Montgomery L. 1988; Use of phylogenetically based hybridization probes for studies of ruminai microbial ecology. Appl. Environ. Microbiol. 54:1079–1084
    [Google Scholar]
  20. Tomioka N., Sugiura M. 1983; The complete nucleotide sequence of a 16S ribosomal RNA from a blue-green alga, Anacystis nidulans. Mol. Gen. Genet. 191:46–50
    [Google Scholar]
  21. Vicini J. L., Brulla W. J., Davis C. L., Bryant M. P. 1987; Quin’s oval and other microbiota in the rumens of molasses-fed sheep. Appl. Environ. Microbiol. 53:1273–1276
    [Google Scholar]
  22. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol. 171:6455–6467
    [Google Scholar]
  23. Wicken A. J., Howard B. H. 1967; On the taxonomic status of “Quin’s oval” organisms. J. Gen. Microbiol. 47:207–211
    [Google Scholar]
  24. Woese C. R. (University of Illinois) 1990 Personal communication
    [Google Scholar]
  25. Woese C. R., Debrunner-Vossbrink B., Oyaizu H., Stackebrandt E., Ludwig W. 1985; Gram-positive bacteria: possible photosynthetic ancestry. Science 229:762–765
    [Google Scholar]
  26. Woese C. R., Gutell R., Gupta R., Noller H. F. 1983; Detailed analysis of the higher order structure of 16S-like ribosomal ribonucleic acid. Microbiol. Rev. 47:621–669
    [Google Scholar]
  27. Woodcock H. M., Lapage G. 1913; On a remarkable type of protistan parasite. Q. J. Microsc. Sci. 59:431–458
    [Google Scholar]
  28. Zhao H., Yang D., Woese C. R., Bryant M. P. 1990; Assignment of Clostridium bryantii to Syntrophospora bryantii gen. nov., comb. nov. on the basis of a 16S rRNA sequence analysis of its crotonate-grown pure culture. Int. J. Syst. Bacteriol. 40:40–44
    [Google Scholar]
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