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Volume 43, Issue 2

sp. nov. from the Green June Beetle (Coleoptera: Scarabaeidae) Free

Abstract

Abstract

Spiroplasma strain CN-5 (T = type strain), isolated from the gut of the cetoniine scarabaeid beetle , was serologically distinct from other spiroplasma species, groups, and subgroups. Cells of strain CN-5 were shown by light microscopy to be helical, motile filaments. Cells in early passages exhibited strong translational motility that tended to be lost in later passages. Electron microscopy showed that the cells were bounded by a single cytoplasmic membrane with no evidence of a cell wall. The organism was not susceptible to penicillin. Strain CN-5 grew well in SM-1, MID, and SP-4 liquid media and on solid SP-4 medium under aerobic or anaerobic conditions. The doubling time at 30°C, the optimum temperature, was 4.3 h. The strain also grew in 1% serum fraction medium. Strain CN-5 produced acid from glucose and catabolized arginine, but did not hydrolyze urea. The guanine-plus-cytosine content of the DNA was 29 ± 1 mol%. The genome size was 1,770 kb (1,186 MDa). Other uncloned isolates obtained from or the cetoniine hermit flower beetle exhibited similar or identical serological patterns. Since no other hosts were discovered in extensive studies, strain CN-5 (previously designated group IX) appears to represent a cluster of relatively host-specific cetoniine beetle-associated strains. Strain CN-5 (= ATCC 33827) is designated the type strain of a new species, .

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Copyright 1993, International Union of Microbiological Societies
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1993-04-01
2024-04-26
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Spiroplasma clarkii sp. nov. from the Green June Beetle (Coleoptera: Scarabaeidae)
Int J Syst Evol Microbiol 43, 261 (1993); https://doi.org/10.1099/00207713-43-2-261
/content/journal/ijsem/10.1099/00207713-43-2-261
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