1887

Abstract

Abstract

Standard growth and isolation methods were used to obtain five new treponema strains in pure culture from deep periodontal pockets. The strains were identified to the species level by various methods, including agglutination, immunofluorescence, a dot blot immunoassay, electron microscopy, gas-liquid chromatography of metabolic volatile fatty acids, and DNA hybridization. Two isolates were strains of the other three were strains of “ a species described in 1925 by Brumpt. Because no type strain was designated for this species previously, the name was not included on the Approved Lists of Bacterial Names and has no current nomenclatural standing. We propose that (ex Brumpt) sp. nov., nom. rev. is a valid and distinct species of the genus and designate strain ATCC 35405 as the type strain and strains ATCC 33520 and ATCC 35404 as reference strains. appears to be the species that is most frequently isolated from periodontal pockets. Unless new isolation and cultivation techniques are introduced, it appears that present technology can yield only isolates belonging to the currently described oral anaerobic spirochete species and that there is little chance of isolating the larger treponemes.

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1993-04-01
2024-12-03
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References

  1. Brondz I., Fiehn N.-E., Olsen I., Sjostrom M. 1991; Multivariate analyses of cellular fatty acids and carbohydrates of 1:2:1 and 2:4:2 spirochetes. Acta Pathol. Microbiol. Immunol. Scand. 99:567–575
    [Google Scholar]
  2. Brumpt E. 1925; Les spirochetoses. 535–578 In Roger G. H., Widal F., Tessier P. J. (ed.) Noveau traite de medecine Masson et Cie; Paris:
    [Google Scholar]
  3. Cheng S.-L., Chan E. C. S. 1983; The routine isolation, growth, and maintenance of the intermediate-size anaerobic oral spirochetes from periodontal pockets. J. Periodontal Res. 18:362–368
    [Google Scholar]
  4. Cheng S.-L., Siboo R., Quee T. Chin, Johnson J. L., Mayberry W. R., Chan E. C. S. 1985; Comparative study of six random oral spirochete isolates. Serological heterogeneity of Treponema denticola. J. Periodontal Res. 20:602–612
    [Google Scholar]
  5. Dawson J. R., Ellen R. P. 1990; Tip-oriented adherence of Treponema denticola to fibronectin. Infect. Immun. 58:3924–3928
    [Google Scholar]
  6. Gornitsky M., Clark D. C., Siboo R., Amsel R., Iugovaz I., Wooley C., Iuliani N., Chan E. C. S. 1991; Clinical documentation and occurrence of putative periodontopathic bacteria in human immunodeficiency virus-associated periodontal disease. J. Periodontol. 62:576–585
    [Google Scholar]
  7. Grenier D., Uitto V.-J., McBride B. C. 1990; Cellular location of a Treponema denticola chymotrypsinlike protease and importance of the protease in migration through the basement membrane. Infect. Immun. 58:347–351
    [Google Scholar]
  8. Holdeman L. V., Cato E. P., Moore W. E. C. (ed.) 1977 Anaerobe laboratory manual, 4th ed.. Anaerobe Laboratory, Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  9. Jacob E., Allen A. L., Nauman R. K. 1979; Detection of oral anaerobic spirochetes in dental plaque by the indirect fluorescent-antibody technique. J. Clin. Microbiol. 10:934–936
    [Google Scholar]
  10. Johnson J. L. 1981; Genetic characterization. 450–472 In Gerhardt P., Murray R. G. E., Costilow R. N., Nester E.W., Wood W. A., Krieg N. R., Phillips G. B. (ed.) Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  11. La Page S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A. (ed.) 1992; International code of nomenclature of bacteria. 1992 Revision American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  12. Leschine S. B., Canale-Parola E. 1980; Rifampin as a selective agent for isolation of oral spirochetes. J. Clin. Microbiol. 12:792–795
    [Google Scholar]
  13. Lindhe J., Ujenberg B. L, Listgarten M. 1980; Some microbiological and histopathological features of periodontal disease in man. J. Periodontol. 51:264–269
    [Google Scholar]
  14. Loesche W. J. 1988; The spirochetes. 263–271 In Newman M. G., Nisengard R. (ed.) Oral microbiology and immunology W. B. Saunders Co.; Philadelphia:
    [Google Scholar]
  15. Loesche W. J., Laughon B. 1982; Role of spirochetes in periodontal disease. 62–75 In Genco R. J., Mergenhagen S. E. (ed.) Host-parasite interactions in periodontal disease American Society for Microbiology; Washington, D.C.:
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  17. Paster B. J., Dewhirst F. E., Weisburg W. G., Tordoff L. A., Fraser G. J., Hespell R. B., Stanton T. B., Zablen L., Mandelco L., Woese C. R. 1991; Phylogenetic analysis of the spirochetes. J. Bacteriol. 173:6101–6109
    [Google Scholar]
  18. Pietrantonio F., Noble P. B., Amsel R., Chan E. C. S. 1988; Locomotory characteristics of Treponema denticola. Can. J. Microbiol. 34:748–752
    [Google Scholar]
  19. Riviere G. R., Wagoner M. A., Baker-Zander S. A., Weisz K. S., Adams D. F., Simonson L., Lukehart S. A. 1991; Identification of spirochetes related to Treponema pallidum in necrotizing ulcerative gingivitis and chronic periodontitis. N. Engl. J. Med. 325:539–543
    [Google Scholar]
  20. Salvador S. L., Syed S. A., Loesche W. J. 1987; Comparison of three dispersion procedures for quantitative recovery of cultivable species of subgingival spirochetes. J. Clin. Microbiol. 25:2230–2232
    [Google Scholar]
  21. Siboo R., Al-Joburi W., Gornitsky M., Chan E. C. S. 1989; Synthesis and secretion of phospholipase C by oral spirochetes. J. Clin. Microbiol. 27:568–570
    [Google Scholar]
  22. Simonson L. G., Goodman C. H., Bial J. J., Morton H. E. 1988; Quantitative relationship of Treponema denticola to severity of periodontal disease. Infect. Immun. 56:726–728
    [Google Scholar]
  23. Simonson L. G., Goodman C. H., Morton H. E. 1990; Quantitative immunoassay of Treponema denticola serovar C in adult periodontitis. J. Clin. Microbiol. 28:1493–1496
    [Google Scholar]
  24. Skerman V. B. D., McGowan V., Sneath P. H. A. (ed.) 1980; Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30:225–420
    [Google Scholar]
  25. Smibert R. M. 1984; Genus III. Treponema Schaudinn 1905. 49–57 In Krieg N. R., Holt J. G. (ed.) Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  26. Tall B. D., Nauman R. K. 1986; Microscopic agglutination and Polyacrylamide gel electrophoresis analyses of oral anaerobic spirochetes. J. Clin. Microbiol. 24:282–287
    [Google Scholar]
  27. Uitto V.-J., Grenier D., Chan E. C. S., McBride B. C. 1988; Isolation of a chymotrypsinlike enzyme from Treponema denticola. Infect. Immun. 56:2717–2722
    [Google Scholar]
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