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Staphylococcus aureus ATCC 12598 and ATCC 25923 were starved of pyridine nucleotides and precursors and then grown in a semidefined medium containing [carbonyl-14C]nicotinamide. Samples of medium from late-exponential-phase and stationary-phase cultures were analyzed for 14C-metabolites. In all cases, V factor was present primarily as NAD.
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