1887

Abstract

Three thermophilic strains, strains P (= DSM 6194) (T = type strain), CALS-1 (= DSM 3870), and Z-517 (= DSM 4774), were characterized chemotaxonomically and compared with five mesophilic strains, GP6 (= DSM 3671), Opfikon (= DSM 2139), FE (= DSM 3013), U, and P. These methanogens were exclusively acetotrophic and had a characteristic sheathed structure. The DNA base compositions of the strains which we studied ranged from 50.3 to 54.3 mol% guanine plus cytosine. The thermophilic strains often had phase-refractive gas vesicles inside their cells. Denaturing electrophoresis of proteins showed that the mesophilic and thermophilic strains formed two distinct groups and that there were differences in protein patterns between the groups. The difference between the thermophiles and mesophiles was also verified by comparing partial 16S rRNA sequences (ca. 30 base differences in ca. 540 bases). On the basis of our results, we propose the name for the three thermophilic strains. The type strain of is strain P (= DSM 6194). We also propose that the name , which was given to strain Z-517 (type strain), should be rejected because of its description, which was based on an enrichment culture, was inadequate.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-42-3-463
1992-07-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/42/3/ijs-42-3-463.html?itemId=/content/journal/ijsem/10.1099/00207713-42-3-463&mimeType=html&fmt=ahah

References

  1. Barker H. A. 1936; Studies upon the methane-producing bacteria. Arch. Mikrobiol. 7:420–438
    [Google Scholar]
  2. Beji A., Izard D., Gavini F., Leclerc H., Leseine-Delstanche M., Krembel J. 1987; A rapid chemical procedure for isolation and purification of chromosomal DNA from gram-negative bacilli. Anal. Biochem. 162:18–23
    [Google Scholar]
  3. Boone D. R. 1991; Strain GP6 is proposed as the neotype strain of Methanothrix soehngeniivp pro synon. Methanothrix conciliiVF and Methanosaeta conciliivp. Request for an opinion. Int. J. Syst. Bacteriol. 41:588–589
    [Google Scholar]
  4. Boone D. R., Whitman W. B. 1988; Proposal of minimal standards for describing new taxa of methanogenic bacteria. Int. J. Syst. Bacteriol. 38:212–219
    [Google Scholar]
  5. Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH 1989 Catalogue of strains, 4th. Gesellschaft fur Biotechnologische Forschung GmbH; Braunschweig, Germany:
    [Google Scholar]
  6. Dolling J., Griffioen A., van Neerven A. R. W., Zevenhuizen L. P. T. M. 1985; Chemical and bacteriological composition of granular methanogenic sludge. Can. J. Microbiol. 31:744–750
    [Google Scholar]
  7. Eggen R., Harmsen H., Geerling A., de Vos W. M. 1989; Nucleotide sequence of a 16S-rRNA encoding gene from the archaebacterium Methanothrix soehngenii. Nucleic Acids Res. 17:9469
    [Google Scholar]
  8. Fathepure B. 1983; Isolation and characterization of an aceticlastic methanogen from a biogas digester. FEMS Microbiol. Lett. 19:151–156
    [Google Scholar]
  9. Huser B. A., Wuhrmann K., Zehnder A. J. B. 1982; Methanothrixsoehngenii gen. nov. sp. nov., a new acetotrophic non-hydrogen-oxidizing methane bacterium. Arch. Microbiol. 132:1–9
    [Google Scholar]
  10. Kamagata Y., Mikami E. 1990; Some characteristics of two morphotypes of Methanothrix soehngenii from mesophilic anaerobic digesters. J. Ferment. Bioeng. 70:272–274
    [Google Scholar]
  11. Kamagata Y., Mikami E. 1991; Isolation and characterization of a novel thermophilic Methanosaeta strain. Int. J. Syst. Bacteriol. 41:191–196
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  13. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc. Natl. Acad. Sci. USA 82:6955–6959
    [Google Scholar]
  14. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A.ed 1975 International code of nomenclature of bacteria. 1976 Revision. American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  15. Lechner K., Wich G., Boeck A. 1985; The nucleotide sequence of the 16S rRNA gene and flanking regions from Methanobacterium formicicum: the phylogenetic relationship between methanogenic and halophilic archaebacteria. Syst. Appl. Microbiol. 6:157–163
    [Google Scholar]
  16. Nishihara M., Morii H., Koga Y. 1987; Structure determination of a quartet of novel tetraether lipids from Methanobacterium thermoautotrophicum. J. Biochem. 101:1007–1015
    [Google Scholar]
  17. Nozhevnikova A. N., Chudina V. I. 1985; Morphology of the thermophilic acetate methane-producing bacterium Methanothrix thermoacetophila sp. nov. Microbiology (Engl. Transi. Mikrobiologiya) 53:618–624
    [Google Scholar]
  18. Ohtsubo S., Miyahara H., Demizu K., Kohno S., Miura I. 1991; Isolation and characterization of new Methanothrix strains. Int. J. Syst. Bacteriol. 41:358–362
    [Google Scholar]
  19. Oyaizu H., Woese C. R. 1985; Phylogenetic relationships among the sulfate respiring bacteria, myxobacteria and purple bacteria. Syst. Appl. Microbiol. 6:257–263
    [Google Scholar]
  20. Patel G. B. 1984; Characterization and nutritional properties of Methanothrix concilii sp. nov., a mesophilic, aceticlastic methanogen. Can. J. Microbiol. 30:1383–1396
    [Google Scholar]
  21. Patel G. B., Sprott G. D. 1990; Methanosaeta concilii gen. nov., sp. nov. (“Methanothrix concilii”) and Methanosaeta thermoacetophila nom. rev., comb. nov. Int. J. Syst. Bacteriol. 40:79–82
    [Google Scholar]
  22. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim. Biophys. Acta 72:619–629
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463–5467
    [Google Scholar]
  24. Söhngen N. L. 1910; Sur le röle du méthane dans la vie organique. Rev. Trav. Chim. 29:238–274
    [Google Scholar]
  25. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol. Lett. 25:125–128
    [Google Scholar]
  26. Touzel J. P., Prensier G., Roustan J. L., Thomas I., Dubourguier H. C., Albagnac G. 1988; Description of a new strain of Methanothrix soehngenii and rejection of Methanothrix concilii as a synonym of Methanothrix soehngenii. Int. J. Syst. Bacteriol. 38:30–36
    [Google Scholar]
  27. Zehnder A. J. B., Huser B. A., Brock T. D., Wuhrmann K. 1980; Characterization of an acetate-decarboxylating, non-hydrogen oxidizing methane bacterium. Arch. Microbiol. 124:111
    [Google Scholar]
  28. Zinder S. H., Anguish T., Lobo A. L. 1987; Isolation and characterization of a thermophilic acetotrophic strain of Methanothrix. Arch. Microbiol. 146:315–322
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-42-3-463
Loading
/content/journal/ijsem/10.1099/00207713-42-3-463
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error