1887

Abstract

subsp. and subsp. are currently differentiated by tolerance to glycine and by their epidemiology. Analysis of DNA by pulsed-field gel electrophoresis, after digestion with the restriction endonucleases I and I, was used to differentiate between the subspecies. All strains presently identified as subsp. had a genomic size of 1.1 Mb, whereas the majority of the subsp. strains had a genomic size of 1.3 Mb. An additional group of strains, which were previously described as subsp. biovar “intermedius” and were able to tolerate higher concentrations of glycine than the rest of the subsp. strains, had an average genome size of 1.5 Mb. We suggest that pulsed-field gel electrophoresis may be useful as an additional aid in the differentiation of strains at the subspecies level.

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1992-07-01
2022-07-04
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