1887

Abstract

We studied 48 isolates that were associated with Lyme borreliosis or were isolated from ticks and identified three DNA relatedness groups by using the S1 nuclease method. The three DNA groups (genospecies) were associated with specific rRNA gene restriction patterns, protein electrophoresis patterns, and patterns of reactivity with murine monoclonal antibodies. Genospecies I corresponded to sensu stricto since it contained the type strain of this species (strain ATCC 35210); this genospecies included 28 isolates from Europe and the United States. Genospecies II was named sp. nov. and included 13 isolates from Europe and Japan. Genospecies III (group VS461) included seven isolates from Europe and Japan.

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1992-07-01
2022-08-17
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References

  1. Adam T., Gassmann G. S., Rasiah C., Göbel U. B. 1991; Phenotypic and genotypic analysis of Borrelia burgdorferi isolates from various sources. Infect. Immun. 59:2579–2585
    [Google Scholar]
  2. Anderson J. F., Doby J. M., Couatannanac’h A., Hyde F. W., Johnson R. C. 1986; Différences antigéniques entre des souches de Borrelia burgdorferi isolées d’Ixodes ricinus en Bretagne. Med. Mal. Infect. 16:171–175
    [Google Scholar]
  3. Barbour A. G. 1984; Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med. 57:521–525
    [Google Scholar]
  4. Barbour A. G. 1988; Plasmid analysis of Borrelia burgdorferi, the Lyme disease agent. J. Clin. Microbiol. 26:475–478
    [Google Scholar]
  5. Barbour A. G. 1989 1. Classification of Borrelia burgdorferi on the basis of plasmid profiles. 1–7 Stanek G., Kristoferitsch W., Pletschette M., Barbour A. G., Flamm H.ed Lyme borreliosis II Gustav Fischer Verlag; Stuttgart, Germany:
    [Google Scholar]
  6. Barbour A. G., Heiland R. A., Howe T. R. 1985; Heterogeneity of major proteins in Lyme disease borreliae: a molecular analysis of North American and European isolates. J. Infect. Dis. 152:478–484
    [Google Scholar]
  7. Bissett M. L., Hill W. 1987; Characterization of Borrelia burgdorferi strains isolated from Ixodes pacificus ticks in California. J. Clin. Microbiol. 25:2296–2301
    [Google Scholar]
  8. Boerlin P., Péter O., Bretz A. G., Postic D., Baranton G., Piffaretti J. C. 1992; Population genetic analysis of Borrelia burgdorferi isolates by multilocus enzyme electrophoresis. Infect. Immun. 60:1677–1683
    [Google Scholar]
  9. Brenner D. J. 1978; Characterization and clinical identification of Enterobacteriaceae by DNA hybridization. Prog. Clin. Pathol. 7:71–117
    [Google Scholar]
  10. Brenner D. J., McWhorter A. C., Leete Knudson J. K., Steigerwalt A. G. 1982; Escherichia vulneris’. a new species of Enterobacteriaceae associated with human wounds. J. Clin. Microbiol. 15:1133–1140
    [Google Scholar]
  11. Burgdorfer W., Barbour A. G., Hayes S. F., Benach J. L., Grunwaldt E., Davis J. P. 1982; Lyme disease—a tick-borne spirochetosis?. Science 216:1317–1319
    [Google Scholar]
  12. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand-specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115:904–911
    [Google Scholar]
  13. Garin C., Bujadoux R. 1922; Paralysie par les tiques. J. Med. Lyon 71:765–767
    [Google Scholar]
  14. Grimont F., Chevrier D., Grimont P. A. D., Lefevre M., Guesdon J. L. 1989; Acetylaminofluorene-labelled ribosomal RNA for use in molecular epidemiology and taxonomy. Res. Microbiol. 140:447–454
    [Google Scholar]
  15. Grimont F., Grimont P. A. D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann. Inst. Pasteur/Microbiol. (Paris) 137B:165–175
    [Google Scholar]
  16. Grimont P. A. D. 1988; Use of DNA reassociation in bacterial classification. Can. J. Microbiol. 34:541–546
    [Google Scholar]
  17. Grimont P. A. D., Popoff M. Y., Grimont F., Coynault C., Lemelin M. 1980; Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures. Curr. Microbiol. 4:325–330
    [Google Scholar]
  18. Hovind-Hougen K., Asbrink E., Stiernstedt G., Steere A. C., Hovmark A. 1986; Ultrastructural differences among spirochetes isolated from patients with Lyme disease and related disorders, and from Ixodes ricinus. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 263:103–111
    [Google Scholar]
  19. Hyde F. W., Johnson R. C. 1984; Genetic relationship of Lyme disease spirochetes to Borrelia, Treponema, and Leptospira spp. J. Clin. Microbiol. 20:151–154
    [Google Scholar]
  20. Johnson R. C., Schmid G. P., Hyde F. W., Steigerwalt A. G., Brenner D. J. 1984; Borrelia burgdorferi sp. nov.: etiological agent of Lyme disease. Int. J. Syst. Bacteriol. 34:496–497
    [Google Scholar]
  21. Kelly R. T. 1984 Borrelia. 57–62 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  22. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 277:680–685
    [Google Scholar]
  23. LeFebvre R. B., Lane R. S., Perng G. C., Brown J. A., Johnson R. C. 1990; DNA and protein analyses of tick-derived isolates of Borrelia burgdorferi from California. J. Clin. Microbiol. 28:700–707
    [Google Scholar]
  24. LeFebvre R. B., Perng G. C., Johnson R. C. 1989; Characterization of Borrelia burgdorferi isolates by restriction endonuclease analysis and DNA hybridization. J. Clin. Microbiol. 27:636–639
    [Google Scholar]
  25. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers 7:503–516
    [Google Scholar]
  26. Péter O. Personal communication
  27. Péter O., Bretz A. G. Polymorphism of outer surface proteins of Borrelia burgdorferi as a tool for the classification. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. in press
    [Google Scholar]
  28. Postic D., Edlinger C., Richaud C., Grimont F., Dufresne Y., Perolat P., Baranton G., Grimont P. A. D. 1990; Two genomic species in Borrelia burgdorferi. Res. Microbiol. 141:465–475
    [Google Scholar]
  29. Rosa P. A., Hogan D., Schwan T. G. 1991; Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi. J. Clin. Microbiol. 29:524–532
    [Google Scholar]
  30. Schmid G. P., Steigerwalt A. G., Johnson S. E., Barbour A. G., Steere A. C., Robinson I. M., Brenner D. J. 1984; DNA characterization of the spirochete that causes Lyme disease. J. Clin. Microbiol. 20:155–158
    [Google Scholar]
  31. Stâlhammar-Carlemalm M., Jenny E., Gern L., Aeschlimann A., Meyer J. 1990; Plasmid analysis and restriction fragment length polymorphisms of chromosomal DNA allow a distinction between Borrelia burgdorferi strains. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 274:28–39
    [Google Scholar]
  32. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandier O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  34. Welsh J., Pretzman C., Postic D., Saint Girons I., Baranton G., McClelland M. 1992; Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups. Int. J. Syst. Bacteriol. 42:370–377
    [Google Scholar]
  35. Wilske B., Preac-Mursic V., Schierz G., Kuhbeck R., Barbour A. G., Kramer M. 1988; Antigenic variability of Borrelia burgdorferi. Ann. N.Y. Acad. Sci. 539:126–143
    [Google Scholar]
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