1887

Abstract

The nucleotide sequence of the 16S rRNA gene of was determined and was compared with the sequence of a related porcine mycoplasma, . While the overall level of DNA-DNA homology was approximately 11%, sequence alignment of the two 16S rRNA genes yielded a homology value of more than 95%, emphasizing the highly conserved nature of the 16S rRNA gene. Multiple sequence alignments with other mollicutes indicated that , and form a subcluster within the fermentans phylogroup, and this subcluster is distinct from the phylogroup. Thus, the three mycoplasmas isolated from porcine respiratory systems exhibit phylogenetic similarities.

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1992-04-01
2023-01-29
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References

  1. Freeman M. J., Armstrong C. H., Sands-Freeman L. L., Lopez-Osuna M. 1984; Serological cross-reactivity of porcine reference antisera to Mycoplasma hyopneumoniae, M. flocculare, M. hyorhinis and M. hyosynoviae indicated by the enzyme-linked immunosorbent assay, complement fixation and indirect hemagglutination tests. Can. J. Comp. Med. 48:202–207
    [Google Scholar]
  2. Friis N. F. 1975; Some recommendations concerning primary isolation of Mycoplasma suipneumoniae and Mycoplasma flocculare.. Nord. Veterinaermed. 27:337–339
    [Google Scholar]
  3. Grau O. 1991; Analyse des gènes ribosomique des mollicutes, application à 1′identitification d’un mollicute non classé et conséquences taxonomiques. Ph.D. thesis Université de Bordeaux II; Bordeaux, France:
    [Google Scholar]
  4. Grau O., Laigret F., Bové J. M. 1990 Analysis of ribosomal RNA genes in two spiroplasmas, one acholeplasma and one unclassified mollicute. 895–897 Stanek G., Cassell G. H., Tully J. G., Whitcomb R. F.ed Recent advances in mycoplasmology Gustav Fischer Verlag; Stuttgart:
    [Google Scholar]
  5. Gross-Bellard M., Oudet P., Chambon P. 1973; Isolation of high molecular weight DNA from mammalian cells. Eur. J. Biochem. 36:32–38
    [Google Scholar]
  6. Huang Y., Stemke G. W. 1989 Mycoplasma flocculare has a rRNA gene organization similar to Mycoplasma hyopneumoniae, abstr. G-32. 153 Abstr. 89th Annu. Meet. Am. Soc. Microbiol 1989 American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  7. Iwami M., Muto A., Yamao F., Osawa S. 1884; Nucleotide sequence of the rmB 16S ribosomal RNA gene from Mycoplasma capricolum.. Mol. Gen. Genet. 196:317–322
    [Google Scholar]
  8. Kirchhoff H., Flossdorf J. 1987; Determination of the guanine-plus-cytosine content of Mollicutes by isopycnic gradient centrifugation. Int. J. Syst. Bacteriol. 37:454–455
    [Google Scholar]
  9. Laigret F., Grau O., Bove J. M. 1990 Comparison of 16S rDNA sequences of various mollicutes. 435–440 Stanek G., Cassell G. H., Tully J. G., Whitcomb R. F.ed Recent advances in mycoplasmology Gustav Fischer Verlag; Stuttgart:
    [Google Scholar]
  10. Laigret F., Hackett K. J., Whitcomb R. F., Tully J. G., Bove J. M. 1990 Ecology and phylogenetics of spiroplasmas: a short review. 55–59 Lesel R.ed Microbiology in poekilotherms Elsevier Science Publications; Amsterdam:
    [Google Scholar]
  11. Lane D. J., Pave D., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analysis. Proc. Natl. Acad. Sci. USA 82:6955–6985
    [Google Scholar]
  12. Loughney K., Lund E., Dahlberg J. E. 1983; Ribosomal RNA precursors of Bacillus subtilis.. Nucleic Acids Res. 11:6709–6721
    [Google Scholar]
  13. Razin S., Freundt E. A. 1984 The mycoplasmas. 740–793 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  14. Rose D. L., Tully J. G., Wittier R. G. 1979; Taxonomy of some swine mycoplasmas: Mycoplasma suipneumoniae Goodwin et al. 1965, a later, objective synonym of Mycoplasma hyopneumoniae Mare and Switzer 1965; and the status of Mycoplasma flocculare Meyling and Friis 1972. Int. J. Syst. Bacteriol. 29:83–91
    [Google Scholar]
  15. Ross R. F. 1982 Mycoplasmal diseases. 535–549 Leman A. D., Glock R. D., Mengeling W. L., Penny R. H. C., Scholl E., Straw B.ed Diseases of swine, 5th. Iowa State University Press; Ames:
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T.ed 1989 Molecular cloning, a laboratory manual, 2nd. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, N.Y:
    [Google Scholar]
  17. Stemke G. W., McIntyre D. J., Roy K. L., Stemler M. E., Robertson J. A. 1985; Guanine-plus-cytosine compositions of and deoxyribonucleic acid hybridization comparisons between Mycoplasma hyopneumoniae and Mycoplasma flocculare.. Int. J. Syst. Bacteriol. 35:527–529
    [Google Scholar]
  18. Taschke C., Herrmann R. 1986; Analysis of transcription and processing signals of the 16S-23S rRNA operon of Mycoplasma hyopneumoniae.. Mol. Gen. Genet. 205:434–441
    [Google Scholar]
  19. Taschke C., Kinkert J., Herrmann R. 1986; Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: the 5S rRNA gene is separated from the 16S and 23S genes. Mol. Gen. Genet. 205:428–433
    [Google Scholar]
  20. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese C. R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J. Bacteriol. 171:6455–6467
    [Google Scholar]
  21. Woese C. R. 1987; Bacterial evolution. Microbiol. Rev. 51:221–271
    [Google Scholar]
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