1887

Abstract

Two cellulolytic clostridia, one thermophilic and the other mesophilic, were isolated and characterized. Cells of the thermophile are gram-negative rods that are motile with lophotrichous flagella and spherical terminal endospores which swell the cells. The optimum growth temperature is 55 to 60°C, with a range of 40 to 65°C. The deoxyribonucleic acid composition is 35 mol% G+C. The name sp. nov. is proposed. The type strain is AS 1.1777. Cells of the mesophile are gram negative and motile with peritrichous flagella and terminal oval or spherical spores which swell the cells. The deoxyribonucleic acid composition is 34 mol% G+C. The name sp. nov. is proposed. The type strain is AS 1.1775. Both AS 1.1777 and AS 1.1775 are deposited in the China Committee for Culture Collection of Microorganisms, Institute of Microbiology, Academia Sinica, Beijing, People’s Republic of China.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-41-2-306
1991-04-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/41/2/ijsem-41-2-306.html?itemId=/content/journal/ijsem/10.1099/00207713-41-2-306&mimeType=html&fmt=ahah

References

  1. Bryant M. P. 1972; Commentary on the Hungate technique for culture of anaerobic bacteria. Am. J. Clin. Nutr. 25:1324–1328
    [Google Scholar]
  2. Cato E. P., George W. L., Finegold S. M. 1986 Genus Clostridium. 1141–1200 Sneath P. H. A., Mair N. S., Sharpe M. E.ed Bergey’s manual of systematic bacteriology 2 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  3. Holdeman L. V., Cato E. P., Moore W. E. C.ed 1977 Anaerobe laboratory manual. , 4th. Anaerobe Laboratory, Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  4. Hungate R. E. 1969 A roll tube method for cultivation of strict anaerobes. 117–132 Norris J. R., Ribbons D. W.ed Methods in microbiology 3B Academic Press, Inc.; New York:
    [Google Scholar]
  5. Jin F., Yamasato K., Toda K. 1988; Clostridium thermocopriae sp. nov., a cellulolytic thermophile from animal feces, compost, soil, and a hot spring in Japan. Int. J. Syst. Bacteriol. 38:279–281
    [Google Scholar]
  6. Madden R. H. 1983; Isolation and characterization of Clostridium stercorarium sp. nov., cellulolytic thermophile. Int. J. Syst. Bacteriol. 33:837–840
    [Google Scholar]
  7. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature for determining the guanine plus cytosine content of DNA. Methods Enzymol. 12B:195–206
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  9. Murray W. D., Hofmann L., Campbell N. L., Madden R. H. 1986; Clostridium lentocellum sp. nov., a cellulolytic species from river sediment containing paper-mill waste. Syst. Appl. Microbiol. 8:181–184
    [Google Scholar]
  10. Petitdemange E., Caillet F., Giallo J., Caudin C. 1984; Clostridium cellulolyticum sp. nov., a cellulolytic, mesophilic species from decayed grass. Int. J. Syst. Bacteriol. 34:155–159
    [Google Scholar]
  11. Ruyet P. L., Dubourguier H. C., Albagnac G., Prensier G. 1985; Characterization of Clostridium thermolaticum sp. nov., a hydrolytic thermophilic anaerobe producing high amounts of lactate. Syst. Appl. Microbiol. 6:196–202
    [Google Scholar]
  12. Sleat R., Mah R. A. 1985; Clostridiumpopuleti sp. nov., a cellulolytic species from a woody-biomass digestor. Int. J. Syst. Bacteriol. 35:160–163
    [Google Scholar]
  13. Sleat R., Mah R. A., Robinson R. 1984; Isolation and characterization of an anaerobic, cellulolytic bacterium, Clostridium cellulovorans sp. nov. Appl. Environ. Microbiol. 48:88–93
    [Google Scholar]
  14. Weimer P. J., Zeikus J. G. 1977; Fermentation of cellulose and cellobiose by Clostridium thermocellum in the absence and presence of Methanobacterium thermoautotrophicum. Appl. Environ. Microbiol. 33:289–297
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-41-2-306
Loading
/content/journal/ijsem/10.1099/00207713-41-2-306
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error