1887

Abstract

isolates from grapevines that were previously characterized as biovar 3 strains were compared with the type strains of the four previously described species of the genus , and Ten grapevine isolates were distinguished from other species phenotypically by a number of biochemical tests and by their reaction with a monoclonal antibody raised to biovar 3. Levels of DNA binding between the type strains of previously described species and three grapevine isolates were estimated by optical measurement of initial DNA renaturation rates. The levels of DNA binding between grapevine isolates were 78 to 92%, and the levels of DNA binding with other type strains ranged from 7 to 47%. Our results indicate that the grapevine isolates are distinct from the type strains of the four previously described species. A new species, , is proposed; the type strain is strain NCPPB 3554.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-40-3-236
1990-07-01
2022-05-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/40/3/ijsem-40-3-236.html?itemId=/content/journal/ijsem/10.1099/00207713-40-3-236&mimeType=html&fmt=ahah

References

  1. Alarcon B., Lopez M. M., Cambra M., Ortiz J. 1987; Comparative study of Agrobacterium biotypes 1, 2 and 3 by electrophoresis and serological methods. J. Appl. Bacteriol. 62:295–308
    [Google Scholar]
  2. Ayers S. H., Rupp P., Johnson W. T. 1919 A study of the alkali-forming bacteria in milk. Bulletin 782. U.S. Department of Agriculture; Washington, D.C:
    [Google Scholar]
  3. Bazzi C., Rosciglione B. 1982; Agrobacterium tumefaciens biovar 3, causal agent of crown gall on Chrysanthemum in Italy. Phytopathol. Z. 103:280–284
    [Google Scholar]
  4. Bergersen F. J. 1961; The growth of Rhizobium in synthetic media. Aust. J. Biol. Sci. 14:349–360
    [Google Scholar]
  5. Bernaerts M. J., De Ley J. 1963; A biochemical test for crown gall bacteria. Nature (London) 197:406–407
    [Google Scholar]
  6. Bishop A. L., Burr T. J., Mittak V. L., Katz B. H. 1989; A monoclonal antibody specific to Agrobacterium tumefaciens biovar 3 and its utilization for indexing grapevine propagation material. Phytopathology 79:995–998
    [Google Scholar]
  7. Brisbane P. G., Kerr A. 1983; Selective media for three biovars of Agrobacterium. J. Appl. Bacteriol. 54:425–431
    [Google Scholar]
  8. Burr T. J., Bishop A. L., Katz B. H., Blanchard L. M., Bazzi C. 1987; A root decay specific to grapevine caused by Agrobacterium tumefaciens and A. radiobacter biovar 3. Phytopathology 77:1424–1427
    [Google Scholar]
  9. Burr T. J., Katz B. H. 1983; Isolation of Agrobacterium tumefaciens biovar 3 from grapevine galls and sap and from vineyard soil. Phytopathology 71:163–165
    [Google Scholar]
  10. De Ley J., Bernaerts M., Rassel A., Guilmot J. 1966; Approach to an improved taxonomy of the genus Agrobacterium. J. Gen. Microbiol. 43:7–17
    [Google Scholar]
  11. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridisation from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  12. De Ley J., Tijtgat R., De Smedt J., Michiels M. 1973; Thermal stability of DNA: DNA hybrids within the genus Agrobacterium. J. Gen. Microbiol. 78:241–252
    [Google Scholar]
  13. EUis J. G., Kerr A., Van Montagu M., Schell J. 1979; Agrobacterium: genetic studies on agrocin production and the biological control of crown gall. Physiol. Plant Pathol. 15:311–319
    [Google Scholar]
  14. Hayward A. C. 1964; Characteristics of Pseudomonas solanacearum. J. Appl. Bacteriol. 27:265–277
    [Google Scholar]
  15. Heberlein G. T., De Ley J., Tijtgat R. 1967; Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium, and Chromobacterium. J. Bacteriol. 94:116–124
    [Google Scholar]
  16. Holmes B., Roberts P. 1981; The classification, identification and nomenclature of agrobacteria. J. Appl. Bacteriol. 50:443–467
    [Google Scholar]
  17. Keane P. J., Kerr A., New P. B. 1970; Crown gall of stone fruit. Identification and nomenclature of Agrobacterium isolates. Aust. J. Biol. Sci. 23:585–595
    [Google Scholar]
  18. Kerr A., Panagopoulos C. G. 1977; Biotypes of Agrobacterium radiobacter var. tumefaciens and their biological control. Phytopathol. Z. 90:172–179
    [Google Scholar]
  19. Kerr A., Young J. M., Panagopoulos C. G. 1978; Genus II. Agrobacterium Conn 1942. N. Z. J. Agric. Res. 21:155–156
    [Google Scholar]
  20. Kersters K., De Ley J. 1984 Genus III. Agrobacterium. 244–254 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  21. Kersters K., De Ley J., Sneath P. H. A., Sackin M. 1973; Numerical taxonomic analysis of Agrobacterium. J. Gen. Microbiol. 78:227–239
    [Google Scholar]
  22. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profiles for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  24. Ophel K., Burr T. J., Magarey P. A., Kerr A. 1988; Detection of Agrobacterium tumefaciens biovar 3 in South Australian grapevine propagation material. Australas. Plant Pathol. 17:61–66
    [Google Scholar]
  25. Panagopoulos C. G., Psallidas P. G. 1973; Characteristics of Greek isolates of Agrobacterium tumefaciens. Smith E. F.Townsend Conn. J. Appl. Bacteriol. 36:233–240
    [Google Scholar]
  26. Panagopoulos C. G., Psallidas P. G., Alivizatos A. S. 1978; Studies on biotype 3 of Agrobacterium tumefaciens var. tumefaciens. 221–228Proceedings of the 4th International Conference on Plant Pathogenic Bacteria, Angers. Institut National de la Recherche AgronomiqueAngers, France
    [Google Scholar]
  27. Petit A., Tempe J., Kerr A., Holsters M., Van Montagu M., Schell J. 1978; Substrate induction of conjugative activity of Agrobacterium tumefaciens Ti plasmids. Nature (London) 271:570–571
    [Google Scholar]
  28. Riker A. J., Banfield W. M., Wright W. H., Keitt G. W., Sagan H. E. 1930; Studies on infectious hairy root of nursery apple trees. J. Agric. Res. 41:507–540
    [Google Scholar]
  29. Rodriguez R. L., Tait R. C. 1983 Recombinant DNA techniques: an introduction. Addison-Wesley, Reading, Mass;
    [Google Scholar]
  30. Ryder M. H., Tate M. E., Kerr A. 1985; Virulence properties of strains of Agrobacterium on the apical and basal surfaces of carrot root discs. Plant Physiol. 77:215–221
    [Google Scholar]
  31. Starr M. P. 1946; The nutrition of phytopathogenic bacteria. II. The genus Agrobacterium. J. Bacteriol. 52:187–194
    [Google Scholar]
  32. Süle S. 1978; Biotypes of Agrobacterium tumefaciens in Hungary. J. Appl. Bacteriol. 44:207–213
    [Google Scholar]
  33. Van Larebeke N., Genetello C., Schell J., Schilperoort R. A., Hermans A. K., Hernalsteens J. P., Van Montagu M. 1975; Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature (London) 255:742–743
    [Google Scholar]
  34. Watson B., Currier T. C., Gordon M. P., Chilton M., Nester E. W. 1975; Plasmid required for virulence of Agrobacterium tumefaciens. J. Bacteriol. 123:255–264
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Truper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  36. White L. O. 1972; The taxonomy of the crown gall organism Agrobacterium tumefaciens and its relationship to rhizobia and other agrobacteria. J. Gen. Microbiol. 72:565–574
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-40-3-236
Loading
/content/journal/ijsem/10.1099/00207713-40-3-236
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error