1887

Abstract

The cellular morphology, colonial morphology, biochemical properties, DNA base compositions, and DNA-DNA homologies of three biovars of were examined. Some differences were found among the three biovars in cellular morphology, colonial morphology, and biochemical properties. The guanine-plus-cytosine contents of DNAs from biovar C strains Fn521 (T = type strain), Fn522, and Fn520 were 30.4, 29.3, and 28.0 mol%, respectively, and the guanine-plus-cytosine contents of DNAs from strains VPI 2891 (biovar A) and VPI 6161 (biovar B) were 31.3 and 32.0 mol%, respectively. Labeled DNA from biovar C strain Fn521 exhibited 96 and 82% relatedness to DNAs from biovar C strains Fn522 and Fn520, respectively; however, it exhibited only about 10% relatedness to DNAs from strains of biovars A and B. Labeled DNAs from strains VPI 2891 and VPI 6161 exhibited more than 70% relatedness to each other, but about 6 to 20% relatedness to DNAs from biovar C strains. Therefore, sp. nov., nom. rev. (ex Prévot 1940) is proposed for biovar C. The type strain is strain Fn521 (= JCM 3722).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-40-1-71
1990-01-01
2024-07-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/40/1/ijsem-40-1-71.html?itemId=/content/journal/ijsem/10.1099/00207713-40-1-71&mimeType=html&fmt=ahah

References

  1. Aalbaek B. 1971; Sphaerophorus necrophorus. A study of 23 strains. Acta Vet. Scand. 12:344–364
    [Google Scholar]
  2. Aulakh G. S., Gallo R. C. 1977; Rauscher-leukemiavirus-related sequences in human DNA: presence in some tissues of some patients with hematopoietic neoplasias and absence in DNA from other tissues. Proc. Natl. Acad. Sci. USA 74:353–357
    [Google Scholar]
  3. Azuma R., Ogimoto K., Suto T. 1962; Anaerobic culture method with steel wool. Jpn. J. Bacteriol. 17:802–806
    [Google Scholar]
  4. Beerens H., Fiévez L., Wattré P. 1971; Observations concemant 7 souches apparent aux espèces Sphaerophorus necrophorus, Sphaerophorus funduliformis et Sphaerophorus pseudonecrophorus. Ann. Inst. Pasteur (Paris) 121:37–41
    [Google Scholar]
  5. Britten R. J., Pavich M., Smith J. 1969; A new method for DNA purification. Carnegie Inst. Wash. Yearbook 68:400–402
    [Google Scholar]
  6. Dowell V. R. Jr., Loper J. C., Hill E. O. 1964; Constancy of deoxyribonucleic acid base composition in the transition of Sphaerophorus necrophorus from bacilli to large bodies. J. Bacteriol. 88:1805–1807
    [Google Scholar]
  7. Emery D. L., Vaughan J. A., Clark B. L., Dufty J. H., Stewart D. J. 1985; Cultural characteristics and virulence of strains of Fusobacterium necrophorum isolated from the feet of cattle and sheep. Aust. Vet. J. 62:43–46
    [Google Scholar]
  8. Fiévez L. 1963 Étude comparée des souches de Sphaerophorus necrophorus isolées chez 1′homme et chez 1′animal. Presess Académiques Européennes; Brussels:
    [Google Scholar]
  9. Harasawa R., Koshimizu K., Pan I. J., Barile M. F. 1985; Genomic and phenotypic analyses of avian Ureaplasma strains. Jpn. J. Vet. Sci. 47:901–909
    [Google Scholar]
  10. Harris J. W., Brown J. H. 1927; Description of a new organism that may be a factor in the causation of puerperal infection. Bull. Johns Hopkins Hosp. 40:203–215
    [Google Scholar]
  11. Johnson J. L. 1973; Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. Int. J. Syst. Bacteriol. 23:308–315
    [Google Scholar]
  12. Maniatis T., Jeffrey A., Kleid D. G. 1975; Nucleotide sequence of the rightward operator of phage λ. Proc. Natl. Acad. Sci. USA 72:1184–1185
    [Google Scholar]
  13. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  14. Miyazato S., Shinjo T., Yago H., Nakamura N. 1978; Fimbriae (pili) detected in Fusobacterium necrophorum. Jpn. J. Vet. Sci. 40:619–621
    [Google Scholar]
  15. Moore W. E. C., Holdeman L. V., Kelley R. W. 1984 Genus Fusobacterium Knorr 1922, 4AL. 631–637 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  16. Page L. R., Krywolap G. N. 1976; Determination of the deoxyribonucleic acid composition and deoxyribonucleic aciddeoxyribonucleic acid hybridization of Fusobacterium fusiforme, Fusobacterium polymorphom, and Leptotrichia buccalis·. taxonomic considerations. Int. J. Syst. Bacteriol. 26:301–304
    [Google Scholar]
  17. Prévot A. R. 1940 Manual de classification et de détermination des bactéries anaérobies. , 1st ed.. Masson et Co.; Paris:
    [Google Scholar]
  18. Rigby P. W., Dieckerman M., Rhodes C., Berg P. 1977; Labeling DNA to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113:237–251
    [Google Scholar]
  19. Shinjo T. 1986; Stability of hemagglutinating and hemolytic activities in Fusobacterium necrophorum biovar A strains. Jpn. J. Vet. Sci. 48:603–604
    [Google Scholar]
  20. Shinjo T., Miyazato S., Kaneuchi C., Mitsuoka T. 1981; Physiological and biochemical characteristics of Fusobacterium necrophorum biovar A and biovar B strains and their deoxyribonucleic acid homology. Jpn. J. Vet. Sci. 43:233–241
    [Google Scholar]
  21. Shinjo T., Yoshitake M., Kiyoyama H., Misawa N., Uchida K. 1981; Liver abscess production in mice by intraportal injection of Fusobacterium necrophorum biovar A strain. Jpn. J. Vet. Sci. 43:919–921
    [Google Scholar]
  22. Tamada H., Harasawa R., Shinjo T. 1985; Isolation of a bacteriophage in Fusobacterium necrophorum. Jpn. J. Vet. Sci. 47:483–186
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-40-1-71
Loading
/content/journal/ijsem/10.1099/00207713-40-1-71
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error