1887

Abstract

A rapid (3-h) arylsulfatase assay for cell suspensions of mycobacteria, in which -nitrophenyl sulfate is used as the substrate, was developed. Arylsulfatase activity was found in cell suspensions of representative strains of , and grown without the substrate in either Middlebrook 7H9 medium containing 0.2% (wt/vol) glucose and 0.05% (vol/vol) Tween 80 or Dubos broth medium, but was absent in cells grown in a low-pH, minimal medium containing 1% (vol/vol) Tween 80 as the sole carbon source. The levels of arylsulfatase activity of representatives of all three species were equal whether the activity was measured at pH 5.5, 6.5, or 7.5 and whether the cells were suspended in phosphate or Tris buffer. The addition of high levels of sulfate (present in the low-pH, Tween 80-containing medium) to Middlebrook 7H9 medium resulted in significantly lower levels of arylsulfatase activity in strains of , but did not affect the levels in either or . The levels of arylsulfatase activity were highest in , intermediate in , and lowest in strains. Polyacrylamide gel electrophoresis of crude extracts from late-log-phase cells of representatives of each species produced activity bands of unique mobility (one in , three in [82, 5, and 13%], and two in [60 and 40%]).

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1990-01-01
2024-10-10
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References

  1. Baess I. 1983; Deoxyribonucleic acid relationships between different serovars of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium scrofulaceum. Acta Pathol. Microbiol. Immunol. Scand. Sect. B 91:201–203
    [Google Scholar]
  2. Brooks R. W., Parker B. C., Graft H., Falkinham J. O. III 1984; Epidemiology of infection by nontuberculous mycobacteria. V. Numbers in eastern United States soils and correlation with soil characteristics. Am. Rev. Respir. Dis. 130:630–633
    [Google Scholar]
  3. Falkinham J. O. III, Parker B. C., Graft H. 1980; Epidemiology of infection by nontuberculous mycobacteria. I. Geographical distribution in the eastern United States. Am. Rev. Respir. Dis. 121:931–937
    [Google Scholar]
  4. George K. L., Falkinham J. O. III 1986; Selective medium for the isolation and enumeration of Mycobacterium aviumintracellulare and M. scrofulaceum. Can. J. Microbiol. 32:10–14
    [Google Scholar]
  5. George K. L., Falkinham J. O. III 1989; Identification of cytoplasmic membrane protein antigens of Mycobacterium avium, M. intracellulare and M. scrofulaceum. Can. J. Microbiol. 35:529–534
    [Google Scholar]
  6. Imaeda T., Barksdale L., Kirchheimer W. F. 1982; Deoxyribonucleic acid of Mycobacterium lepraemurium•. its genome size, base ratio, and homology with those of other mycobacteria. Int. J. Syst. Bacteriol. 32:456–458
    [Google Scholar]
  7. Kubica G. P. 1973; Differential identification of mycobacteria. VII. Key features for identification of clinically significant mycobacteria. Am. Rev. Respir. Dis. 107:9–21
    [Google Scholar]
  8. Kubica G. P., Beam R. E. 1961; The arylsulfatase activity of acid-fast bacilli. II. The differentiation of Mycobacterium avium from the unclassified group III nonphotochromogenic mycobacteria. Am. Rev. Respir. Dis. 83:733–736
    [Google Scholar]
  9. Kubica G. P., Rigdon A. L. 1961; The arylsulfatase activity of acid-fast bacilli. III. Preliminary investigation of rapidly growing acid-fast bacilli. Am. Rev. Respir. Dis. 83:737–740
    [Google Scholar]
  10. Kubica G. P., Vestal A. L. 1961; The arylsulfatase activity of acid-fast bacilli. I. Investigation of activity of stock cultures of acid-fast bacilli. Am. Rev. Respir. Dis. 83:728–732
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr O. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chern. 193:265–275
    [Google Scholar]
  12. Tsukamura M. 1966; Adansonian classification of mycobacteria. J. Gen. Microbiol. 45:253–273
    [Google Scholar]
  13. Tsukamura M. 1967; Identification of mycobacteria. Tubercle 48:311–338
    [Google Scholar]
  14. Tsukamura M. 1976; Numerical classification of slowly growing mycobacteria. Int. J. Syst. Bacteriol. 26:409–420
    [Google Scholar]
  15. Tsukamura M. 1981; A review of the methods of identification and differentiation of mycobacteria. Rev. Infect. Dis. 3:841–861
    [Google Scholar]
  16. Tsukamura M. 1985; Numerical identification of slowly growing mycobacteria. Microbiol. Immunol. 29:1039–1050
    [Google Scholar]
  17. Tsukamura M., Mizuno S., Murata H., Nemoto H., Yugi H. 1982; Relationship between serotype and certain biological and biochemical characteristics of strains of the Mycobacterium avium and Mycobacterium intracellulare complex. Microbiol. Immunol. 26:871–875
    [Google Scholar]
  18. Wayne L. G. 1961; Recognition of My cobacterium fortuitum by means of a three-day phenolphthalein sulfatase test. Am. J. Clin. Pathol. 36:185–187
    [Google Scholar]
  19. Wayne L. G., Juarez W. J., Nichols E. G. 1958; Arylsulfatase activity of aerobic actinomycetales. J. Bacteriol. 75:367–368
    [Google Scholar]
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