1887

Abstract

The electrophoretic mobilities of glutamate dehydrogenase and 2-oxoglutarate reductase were compared for three reference strains and 30 human, oral isolates of Both enzymes allowed the same strains to be grouped into three electrophoretic clusters, designated groups Fn-1, Fn-2, and Fn-3. Group Fn-1 contained the type strain of , strain ATCC 25586, and nine clinical isolates. Group Fn-2 comprised 20 strains and appeared to contain the strains of that are isolated most commonly from oral cavities. Strain NCTC 10953 (formerly “”) was a member of this cluster. Strains of group Fn-3 were rarely isolated; this group contained three isolates and reference strain NCTC 11362, which was listed previously as “.” The deoxyribonucleic acid (DNA) base compositions of all strains were between 25 and 27 mol% guanine plus cytosine. Under optimal conditions of DNA-DNA hybridization, all of the strains exhibited high levels of DNA homology (73 to 99%) to the three reference DNA probes belonging to groups Fn-1, Fn-2, and Fn-3. However, under stringent DNA hybridization conditions there was evidence of more genetic homogeneity within each group.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-39-4-467
1989-01-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/39/4/ijs-39-4-467.html?itemId=/content/journal/ijsem/10.1099/00207713-39-4-467&mimeType=html&fmt=ahah

References

  1. Calhoon D. A., Mayberry W. R., Slots J. 1983; Cellular fatty and soluble protein profiles of oral fusobacteria. J. Dent. Res. 62:1181–1185
    [Google Scholar]
  2. Cato E. P., Moore L. V., Moore W. E. C. 1983; Fusobacterium alocis sp. nov. and Fusobacterium sulci sp. nov. from the human gingival sulcus. Int. J. Syst. Bacteriol. 35:475–477
    [Google Scholar]
  3. Finegold S. M., George W. L., Mulligan M. E. 1985; Disease-a-month. 3–69 Cotstonas N. J. Jr. Anaerobic infections, part 1 31 DM Yearbook Medical Publisher; Chicago:
    [Google Scholar]
  4. Gharbia S. E., Shah H. N. 1988; Characteristics of glutamate dehydrogenase, a new diagnostic marker for the genus Fusobacterium. J. Gen. Microbiol. 134:327–332
    [Google Scholar]
  5. Hine M. K., Berry G. P. 1937; Morphological and cultural studies on the genus Fusiformis. J. Bacteriol. 34:517–534
    [Google Scholar]
  6. Hoffman H. 1957; Genus Fusobacterium (Knorr 1922). 436–440 Breed R. S., Murray E. G. D., Smith N. Bergey’s manual of determinative bacteriology, 7th ed.. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  7. Hofstad T. 1979; Serological responses to antigens of Bacte- roidaceae. Microbiol. Rev. 43:103–115
    [Google Scholar]
  8. Holdeman L. V., Moore W. E. C. 1972 Anaerobe laboratory manual Anaerobe Laboratory, Virginia Polytechnic Institute and State University; Blacksburg:
    [Google Scholar]
  9. Jantzen E., Hofstad T. 1981; Fatty acids of Fusobacterium species: taxonomic implications. J. Gen. Microbiol. 123:163–171
    [Google Scholar]
  10. Kelly R. B., Cozzarelli N. R., Deutscher M. P., Lehman I. R., Kornberg A. 1970; Enzymatic synthesis of deoxyribonucleic acid. XXXII. Replication of complex deoxyribonucleic acid by polymerase at a single strand break. J. Biol. Chem. 245:39–45
    [Google Scholar]
  11. Knorr M. 1922; Uber die fusospirillane Symbiose, die Gattung Fusobacterium (K. B. Lehman) und Spirrilium sputigenum (Zugleich ein Beitrag Zur Bakteriologie der Mundhohle). I. Mitteilung: die Epidemiologie der fusospirillaren Symbiose, besonders der Flaut-Vincentschen Angina. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 89:531–545
    [Google Scholar]
  12. Knorr M. 1923; Uber die fusospirillane Symbiose, die Gattung Fusobacterium (K. B. Lehman) und Spirrilium sputigenum. (Zugleich ein Beitrag Zur Bakteriologie der Mundhohle). It. Mitteilung: die Gattung Fusobacterium. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. 89:4–22
    [Google Scholar]
  13. Love D. N., Cato E. P., Johnson J. L., Jones R. F., Bailey M. 1987; Deoxyribonucleic acid hybridization among strains of fusobacteria isolated from soft tissue infections of cats: comparison with human and animal type strains from oral and other sites. Int. J. Syst. Bacteriol. 37:23–26
    [Google Scholar]
  14. Miyagawa E., Azuma R., Suto T. 1981; Peptidoglycan composition of Gram-negative obligatory anaerobic rods. J. Gen. Appl. Microbiol. 27:199–208
    [Google Scholar]
  15. Moore W. E. C., Holdeman L. V. 1974; Genus Fusobacterium (Knorr, 1922). 404–418 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8th ed.. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  16. Moore W. E. C., Holdeman L. V., Kelley W. 1984; Genus II. Fusobacterium Knorr 1922, 4AL . 631–637 Krieg N. R. Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  17. Murdoch D. A., Mitchelmore I. J., Nash R. A., Hardie J. M., Tabaqchali S. 1988; Preformed enzyme profiles of reference strains of gram-positive anaerobic cocci. J. Med. Microbiol. 27:65–70
    [Google Scholar]
  18. Shah H. N., Gharbia S. E., Al-Jalilii T. A. R., Nash R. A., Seddon S. V. 1987; Enzymes of diagnostic importance within the Bacteroidaceae: use as possible ecological markers. Microbiol. Health Dis. 1:115–121
    [Google Scholar]
  19. Shah H. N., van Steenbergen T. J. M., Hardie J. M., deGraaff J. 1982; DNA base composition, DNA-DNA reassociation and isoelectric-focusing of proteins of strains designated Bacteroides oralis. FEMS Microbiol. Lett. 13:125–130
    [Google Scholar]
  20. Shah H. N., Williams R. A. D. 1982; Dehydrogenase patterns in the taxonomy of Bacteroides. J. Gen. Microbiol. 128:2955–2965
    [Google Scholar]
  21. Shah H. N., Williams R. A. D., Bowden G. H., Hardie J. M. 1976; Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites. J. Appl. Bacteriol. 41:473–492
    [Google Scholar]
  22. Shaw C. R. 1965; Electrophoretic variation in enzymes. Science 149:936–942
    [Google Scholar]
  23. Slots J., Potts T. V., Mashimo P. A. 1983; Fusobacterium periodonticum, a new species from the human oral cavity. J. Dent. Res. 62:960–963
    [Google Scholar]
  24. Tanner A. C. R., Haffer C., Bratthail G. T., Visconti R. A., Socransky S. S. 1979; A study of the bacteria associated with advancing periodontitis in man. J. Clin. Periodontol. 6:278–307
    [Google Scholar]
  25. Van Palenstein Helderman W. H. 1975; Total viable count and differential count of Vibrio (Campylobacter) sputorum, Fusobacterium nucleatum, Bacteroides ocharaceus and Veillonella in the inflamed and non-inflamed human gingival crevice. J. Periodontal Res. 10:294–305
    [Google Scholar]
  26. Vasstrand E. N., Jansen H. B., Miron T., Hofstad T. 1981; Composition of peptidoglycan in Bacteroidaceae: determination and distribution of lanthionine. Infect. Immun. 36:114–122
    [Google Scholar]
  27. Veillon A., Zuber A. 1898; Recherches sur quelques microbes strictement anaerobies et leur role en pathologie. Arch. Med. Exp. Anat. Pathol. 10:517–545
    [Google Scholar]
  28. Williams B. L., Pantalone R. M., Sherris J. C. 1976; Subgingival microflora and periodontitis. J. Periodontal Res. 11:1–18
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-39-4-467
Loading
/content/journal/ijsem/10.1099/00207713-39-4-467
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error