We studied the purine enzyme activities in dialyzed cytoplasmic extracts from the following eight species, representing four genera, of FH (T = type strain) and M129, PG-11, PG-21 and 1620, G-37, J, T960, Maroc-R8A2, and 5LA. In an investigation of purine nucleoside kinase activity we also included 13408, 10144, 13428, 1612, 1184, and Botte. All of these species except had purine phosphoribosyltransferase activity for adenine, hypoxanthine, and guanine; had only adenine phosphoribosyltransferase activity. All of the organisms had nucleoside phosphorylase activity which used either ribose 1-phosphate or deoxyribose 1-phosphate and adenine, hypoxanthine, or guanine for the synthesis of nucleosides and adenosine, deoxyadenosine, guanosine, deoxyguanosine, inosine, or deoxyinosine in the reverse direction. All had 5′-nucleotidase activity for adenosine monophosphate, deoxyadenosine monophosphate, inosine monophosphate, or guanosine monophosphate. Only 1620, 13408, 10144, and 13428, , and had pyrophosphate-dependent nucleoside kinase activity. Only had nucleoside kinase activity with adenosine triphosphate and deoxyguanosine. We studied pyrimidine enzyme activities in all of the species except and . All of the species assayed had thymidine, thymidylate, and deoxycytidine kinase and thymidine and uridine phosphorylase activities. All of the spp. had deoxycytotidine monophosphate and cytidine-deoxycytidine deaminase activities. All of the spp. and lacked deoxyuridine triphosphatase activity. lacked deoxycytidine monophosphate deaminase activity, but otherwise it resembled all of the spp. and differed from each other and from spp. and in the patterns of pyrimidine enzyme activities. Pyrophosphate-dependent nucleoside kinase activity was the most variably detected activity. None of the spp. except four of eight strains of had this kinase activity. Likewise, did not have the pyrophosphate-dependent nucleoside kinase activity; however, and did have this enzyme activity. The absence of deoxyuridine triphosphatase activity in all spp. may be related to their proposed rapid evolution and the relative lack of conserved sequences in their 5S ribosomal ribonucleic acids.


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