1887

Abstract

sp. strain TWAR is an important cause of acute respiratory disease in humans. To determine the genetic relatedness of the TWAR organism to and , solution hybridization followed by SI nuclease assays were performed, and guanine-plus-cytosine (G+C) contents were determined. Deoxyribonucleic acid (DNA) preparations from three TWAR isolates were radioactively labeled and hybridized against DNAs from three serovars of and five strains of . Reciprocal experiments were also done by using radioactively labeled and DNAs against TWAR DNA. In all cases, the degree of DNA relatedness between TWAR and the two species was 10% or less. Within the group of TWAR isolates, there was 94% or greater sequence similarity. The G+C values for TWAR were intermediate between the G+C values for and C. psittaci. The degrees of DNA relatedness among the strains within the species ranged from 20 to 100%.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-38-3-265
1988-07-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/38/3/ijsem-38-3-265.html?itemId=/content/journal/ijsem/10.1099/00207713-38-3-265&mimeType=html&fmt=ahah

References

  1. Baker J. A. 1944; A virus causing pneumonia in cats and producing elementary bodies. J. Exp. Med. 79:159–171
    [Google Scholar]
  2. Campbell L. A., Kuo C.-C., Grayston J. T. 1987; Characterization of the new Chlamydia agent, TWAR, as a unique organism by restriction endonuclease analysis and DNA-DNA hybridization. J. Clin. Microbiol. 25:1911–1916
    [Google Scholar]
  3. Chi E. Y., Kuo C.-C., Grayston J. T. 1987; Unique ultrastructure in the elementary body of Chlamydia sp. strain TWAR. J. Bacteriol. 169:3757–3763
    [Google Scholar]
  4. Crosa J. H., Brenner D. J., Falkow S. 1973; Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J. Bacteriol. 115:904–911
    [Google Scholar]
  5. Francis T. Jr., Magill T. O. 1938; An unidentified virus producing acute meningitis and pneumonia. J. Exp. Med. 68:147–160
    [Google Scholar]
  6. Gerloff R. K., Ritter D. H., Watson R. O. 1966; DNA homology between meningopneumonitis agent and related organisms. J. Infect. Dis. 116:197–202
    [Google Scholar]
  7. Gerloff R. K., Ritter D. B., Watson R. O. 1970; Studies on the thermal denaturation of DNA from various chlamydiae. J. Infect. Dis. 121:65–69
    [Google Scholar]
  8. Gordon F. B., Quan A. L. 1965; Occurrence of glycogen in inclusions of the psittacosis-lymphogranuloma venereum trachoma agents. J. Infect. Dis. 115:186–196
    [Google Scholar]
  9. Grayston J. T., Kuo C.-C., Wang S.-P., Altman J. 1986; A new Chlamydia psittaci strain, called TWAR, from acute respiratory tract infection. N. Engl. J. Med. 315:161–168
    [Google Scholar]
  10. Herring A. J., McClenaghan M., Aitken I. D. 1986 Nucleic acid techniques for strain differentiation and detection of Chlamydia psittaci. 578–579 Oriel D., Ridgway G., Schachter J., Taylor-Robinson D., Ward M.ed Chlamydial infection Cambridge University Press; Cambridge, England:
    [Google Scholar]
  11. Johnson J. L. 1981 Genetic characterization. 450–472 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B.ed Manual of methods for general bacteriology American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  12. Johnson J. L. 1984 Nucleic acids in bacterial classification. 8–11 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore.:
    [Google Scholar]
  13. Kazdan J. T., Schachter J., Okumoto M. 1967; Inclusion conjunctivitis in the guinea pig. Am. J. Opthamol. 64:116–124
    [Google Scholar]
  14. Kingsbury D. T., Wiess E. 1968; Lack of deoxyribonucleic acid homology between species of the genus Chlamydia. J. Bacteriol. 96:1421–1423
    [Google Scholar]
  15. Kuo C.-C., Chen H.-H., Wang S.-P., Grayston J. T. 1986; Identification of a new group of Chlamydia psittaci strains called TWAR. J. Clin. Microbiol. 24:1034–1037
    [Google Scholar]
  16. Kuo C.-C., Kenny G. E., Wang S.-P. 1971 Trachoma and psittacosis antigens in agar gel double immunodiffusion. 113–123 Nichols R. L.ed Trachoma and related disorders caused by chlamydial agents Excerpta Medica; Amsterdam:
    [Google Scholar]
  17. Kuo C.-C., Wang S.-P., Grayston J. T. 1977 Growth of trachoma organisms in HeLa 229 cell culture. 328–336 Hobson D., Holmes K. K.ed Nongonococcal urethritis and related infections American Society for Microbiology; Washington, D.C:
    [Google Scholar]
  18. Maniatis T., Fritsch E. F., Sambrook J. 1983 Molecular cloning, a laboratory manual Cold Spring Harbor Laboratory. Cold Spring Harbor; N.Y:
    [Google Scholar]
  19. Marrie T. J., Grayston J. T., Wang S.-P., Kuo C.-C. 1987; Pneumonia due to TWAR, a new Chlamydia strain. Ann. Intern. Med. 106:507–511
    [Google Scholar]
  20. McClenahgan M., Herring A. J., Aitken I. D. 1984; Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis. Infect. Immun. 45:384–389
    [Google Scholar]
  21. Moulder J. W., Hatch T. P., Kuo C.-C., Schachter J., Storz J. 1984 Chlamydia. Jones, Rake, and Stears 1945, 55. 729–739 Krieg N. R., Holt J. G.ed Bergey’s manual of systematic bacteriology 1 The Williams & Wilkins Co.; Baltimore.:
    [Google Scholar]
  22. Page L. A. 1966; Revision of the family Chlamydiaceae Rake (Rickettsiales)’. unification of the psittacosis-lymphogranuloma venereum-trachoma group of organisms in the genus Chlamydia Jones, Rake, and Stearns, 1945. Int. J. Syst. Bacteriol. 16:223–252
    [Google Scholar]
  23. Page L. A. 1968; Proposal for the recognition of two species in the genus Chlamydia Jones, Rake, and Stearns, 1945. Int. J. Syst. Bacteriol. 18:51–66
    [Google Scholar]
  24. Perez-Martinez J. A., Storz J. 1985; Antigenic diversity of Chlamydia psittaci of mammalian origin determined by microimmunofluorescence. Infect. Immun. 50:905–910
    [Google Scholar]
  25. Saikku P., Wang S.-P., Keemola M., Brander E., Rusanen E., Grayston J. T. 1985; An epidemic of mild pneumonia due to an unusual strain of Chlamydia psittaci. J. Infect. Dis. 151:832–839
    [Google Scholar]
  26. Schachter J., Meyer K. F. 1969; Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J. Bacteriol. 99:636–638
    [Google Scholar]
  27. Wang S.-P., Grayston J. T. 1971 Classification of TRIC and related strains with microimmunofluorscence. 305–321 Nichols R. L.ed Trachoma and related disorders caused by chlamydial agents Excerpta Medica; Amsterdam:
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandier O., Krichesvky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Truper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol. 37:463–464
    [Google Scholar]
  29. Weiss E., Schramek S., Wilson N. N., Newman L. W. 1970; Deoxyribonucleic acid heterogeneity between human and murine strains of Chlamydia trachomatis. Infect. Immun. 2:24–28
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-38-3-265
Loading
/content/journal/ijsem/10.1099/00207713-38-3-265
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error