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The guanine-plus-cytosine (G+C) ratio of the deoxyribonucleic acid (DNA) was determined for 7 Acholeplasma and 24 Mycoplasma species by a new method that combines a micro-scale preparation of DNA with isopycnic gradient centrifugation. For three Acholeplasma and seven Mycoplasma species, the results were compared with values obtained by other investigators.
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