1887

Abstract

Deoxyribonucleic acid (DNA) base composition and DNA base sequence relatedness comparisons were used for species delineation in the genus . Base composition values separated the members of the genus into three groups. The groups were further subdivided by comparing base sequences by using DNA/DNA renaturation experiments. Two DNA homology groups were identified. The first group included , and three anamorphs (, and ); the members of this group exhibited ≥90% DNA base sequence complementarity. The second group consisted of , and ; various pairs of these yeasts shared 64 to 98% of their DNA sequences. The two groups were only distantly related to each other (≤15% DNA base sequence complementarity). The other species appear to be unique, not being closely related to either of the two homology groups or to one another. Relationships deduced from comparisons of DNAs agreed well with those deduced by other workers from immunological comparisons of exo-β-glucanases and from isoenzyme analysis but were only in partial agreement with a taxonomic arrangement made on the basis of mating studies. We propose recognition of the following species: , and .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-37-4-371
1987-10-01
2022-05-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/37/4/ijsem-37-4-371.html?itemId=/content/journal/ijsem/10.1099/00207713-37-4-371&mimeType=html&fmt=ahah

References

  1. Bicknell J. N., Douglas H. C. 1970; Nucleic acid homologies among species of Saccharomyces. J. Bacteriol. 101:505–512
    [Google Scholar]
  2. Brenner D. J., Fanning G. R., Rake A. V., Johnson K. E. 1969; Batch procedure for thermal elution of DNA from hydroxyapatite. Anal. Biochem. 28:447–459
    [Google Scholar]
  3. Carson H. L., Knapp E. P., Phaff H. J. 1956; The yeast flora of the natural breeding sites of some species of Drosophila. Ecology 37:538–544
    [Google Scholar]
  4. Commerford S. L. 1971; Iodination of nucleic acids in vitro. Biochemistry 10:1993–2000
    [Google Scholar]
  5. Dobzhansky T. 1951 Genetics and the origin of species, 3rd.179–253 Columbia University Press; New York:
    [Google Scholar]
  6. Dobzhansky T. 1976 Organismic and molecular aspects of species formation. 95–105 Ayala F. J.ed Molecular evolution Sinauer Associates, Inc.; Sunderland, Mass:
    [Google Scholar]
  7. Dobzhansky T., Ayala F. J., Stebbins G. L., Valentine J. W. 1977 Evolution. 165–232 W. H. Freeman & Co.; New York:
    [Google Scholar]
  8. Douglas H. C., Grindall D. P., Talbott H. 1969; Electrophoretic variants of phosphoglucomutase in Saccharomyces species. J. Bacteriol. 99:287–290
    [Google Scholar]
  9. Fell J. W. 1961; A new species of Saccharomyces isolated from a subtropical estuary. Antonie van Leeuwenhoek J. Microbiol. 27:27–30
    [Google Scholar]
  10. Fiol J. B., Poncet S. 1980; Systématique du genre Kluyveromyces-. hybridation ADN-ADN. Mycopathologia 70:13–23
    [Google Scholar]
  11. Fuson G. B., Price C. W., Phaff H. J. 1980; Deoxyribonucleic acid base sequence relatedness among strains of Pichia ohmeri that produce dimorphic ascospores. Int. J. Syst. Bacteriol. 30:217–219
    [Google Scholar]
  12. Grant V. 1977 Organismic evolution. 177–185 W. H. Freeman & Co.; New York:
    [Google Scholar]
  13. Henninger W., Windisch S. 1976; Kluyveromyces blattae sp. n., eine neue vielsporige Hefe aus Blatta orientalis. Arch. Microbiol. 109:153–156
    [Google Scholar]
  14. Holzschu D. L., Phaff H. J. 1982 Taxonomy and evolution of some ascomycetous cactophilic yeasts. 127–141 Barker J. S. F., Starmer W. T.ed Ecological genetics and evolution: the cactus-yeast-Drosop/ii/a model system Academic Press, Inc.; Sydney, Australia:
    [Google Scholar]
  15. Humphreys G. O., Willshaw G. A., Anderson E. S. 1975; A simple method for the preparation of large quantities of pure plasmid DNA. Biochim. Biophys. Acta 383:457–463
    [Google Scholar]
  16. Johannsen E. 1980; Hybridization studies within the genus Kluyveromyces van der Walt emend, van der Walt. Antonie van Leeuwenhoek J. Microbiol. 46:177–189
    [Google Scholar]
  17. Johannsen E., van der Walt J. P. 1978; Interfertility as basis for the delimitation of Kluyveromyces marxianus. Arch. Microbiol. 118:45–48
    [Google Scholar]
  18. Kurtzman C. P. 1984 Pichia Hansen. 295–378 Kreger-van Rij N. J. W.ed The yeasts: a taxonomic study, 3rd. Elsevier Science Publishers B. V.; Amsterdam:
    [Google Scholar]
  19. Kurtzman C. P., Phaff H. J. 1987 Molecular taxonomy. 63–94 Rose A. H., Harrison J. S.ed The yeasts, 2nd. 1 Academic Press, Inc. (London), Ltd.; London:
    [Google Scholar]
  20. Kurtzman C. P., Phaff H. J., Meyer S. A. 1983 Nucleic acid relatedness among yeasts. 139–166 Spencer J. F. T., Spencer D. M., Smith A. R. W.ed Yeast genetics: fundamental and applied aspects Springer-Verlag; New York:
    [Google Scholar]
  21. Kurtzman C. P., Smiley M. J., Johnson C. J. 1980; Emendation of the genus Issatchenkia Kudriavzev and comparison of species by deoxyribonucleic acid reassociation, mating reaction, and ascospore ultrastructure. Int. J. Syst. Bacteriol. 30:503–513
    [Google Scholar]
  22. Lachance M.-A., Phaff H. J. 1979; Comparative study of molecular size and structure of exo-p-glucanases from Kluyveromyces and other yeast genera: evolutionary and taxonomic implications. Int. J. Syst. Bacteriol. 29:70–78
    [Google Scholar]
  23. Martini A. 1973; Ibridazioni DNA/DNA tra specie di lieviti del genere Kluyveromyces. Ann. Fac. Agrar. Univ. Perugia 28:157–171
    [Google Scholar]
  24. Martini A., Phaff H. J. 1973; The optical determination of DNA/DNA homologies in yeasts. Ann. Microbiol. (Milan) 23:59–68
    [Google Scholar]
  25. Martini A., Phaff H. J., Douglass S. A. 1972; Deoxyribonucleic acid base composition of species in the yeast genus Kluyveromyces van der Walt emend, van der Walt. J. Bacteriol. 111:481–487
    [Google Scholar]
  26. Meyer S. A., Ahearn D. G., Yarrow D. 1984 Candida Berkhout. 585–844 Kreger-van Rij N. J. W.ed The yeasts: a taxonomic study, 3rd. Elsevier Science Publishers B. V.; Amsterdam:
    [Google Scholar]
  27. Phaff H. J. 1980 The species concept in yeast: physiologic, morphologic, genetic, and écologie parameters. 635–643 Stewart G. G., Russell I.ed Current developments in yeast research Pergamon Press; Toronto:
    [Google Scholar]
  28. Poncet S. 1973; Taxonomie numérique du genre Kluyveromyces. Mycopathol. Mycol. Appl. 51:267–281
    [Google Scholar]
  29. Poncet S., Fiol J.-B. 1972; Taxonomy of Kluyveromyces’. evaluation of DNA base composition. Antonie van Leeuwenhoek J. Microbiol. 38:145–152
    [Google Scholar]
  30. Price C. W., Fuson G. B., Phaff H. J. 1978; Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia. Microbiol. Rev. 42:161–193
    [Google Scholar]
  31. Schildkraut C. L., Marmiir J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol. 4:430–443
    [Google Scholar]
  32. Sidenberg D. G., Lachance M.-A. 1983; Speciation, species delineation, and electrophoretic isoenzyme patterns of the type strains of Kluyveromyces van der Walt emend, van der Walt. Int. J. Syst. Bacteriol. 33:822–828
    [Google Scholar]
  33. Sidenberg D. G., Lachance M.-A. 1986; Electrophoretic isoenzyme variation in Kluyveromyces populations and revision of Kluyveromyces marxianus (Hansen) van der Walt. Int. J. Syst. Bacteriol. 36:94–102
    [Google Scholar]
  34. van der Walt J. P. 1956; Kluyveromyces–a new yeast genus of the Endomycetales. Antonie van Leeuwenhoek J. Microbiol. 22:265–272
    [Google Scholar]
  35. van der Walt J. P. 1956; The yeast Kluyveromyces africanus nov. spec, and its phylogenetic significance. Antonie van Leeuwenhoek J. Microbiol. 22:321–326
    [Google Scholar]
  36. van der Walt J. P. 1965; The emendation of the genus Kluyveromyces v. d. Walt. Antonie van Leeuwenhoek J. Microbiol. 31:341–348
    [Google Scholar]
  37. van der Walt J. P. 1970 Kluyveromyces van der Walt emend. van der Walt. 316–378 Lodder J.ed The yeasts: a taxonomic study, 2nd. Elsevier/North-Holland Publishing Co.; Amsterdam:
    [Google Scholar]
  38. van der Walt J. P., Johannsen E. 1979; A comparison of interfertility and in vitro DNA-DNA reassociation as criteria for speciation in the genus Kluyveromyces. Antonie van Leeuwenhoek J. Microbiol. 45:281–291
    [Google Scholar]
  39. van der Walt J. P., Johannsen E. 1984 Kluyveromyces van der Walt emend, van der Walt. 224–251 Kreger-van Rij N. J. W.ed The yeasts: a taxonomic study, 3rd. Elsevier Science Publishers B. V.; Amsterdam:
    [Google Scholar]
  40. Vaughan Martini A., Martini A. 1985; Perfect-imperfect relationships within the yeast genus Kluyveromyces. Ann. Microbiol. (Milan) 35:93–97
    [Google Scholar]
  41. Vaughan Martini A., Martini A. 1987; Taxonomic revision of the yeast genus Kluyveromyces by nuclear deoxyribonucleic acid reassociation. Int. J. Syst. Bacteriol. 37:380–385
    [Google Scholar]
  42. Wetmur J. G., Davidson N<. 1968; Kinetics of renaturation of DNA. J. Mol. Biol. 31:349–370
    [Google Scholar]
  43. Wickerham L. J., Burton K. A. 1956; Hybridization studies involving Saccharomyces lactis and Zygosaccharomyces ashbyi. J. Bacteriol. 71:290–295
    [Google Scholar]
  44. Wickerham L. J., Burton K. A. 1956; Hybridization studies involving Saccharomyces fragilis and Zygosac-charomyces dobzhanskii. J. Bacteriol. 71:296–302
    [Google Scholar]
  45. Wickerham L. J., Kurtzman C. P., Herman A. I. 1970; Sexual reproduction in Candida lipolytica. Science 167:1141
    [Google Scholar]
  46. Yamada Y., Nojiri M., Matsuyama M., Kondo K. 1976; Coenzyme Q system in the classification of the asco-sporogenous yeast genera Debaryomyces, Saccharomyces, Kluyveromyces, and Endomycopsis. J. Gen. Appl. Microbiol. 22:325–337
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-37-4-371
Loading
/content/journal/ijsem/10.1099/00207713-37-4-371
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error