1887

Abstract

The taxonomy of two groups (designated as 3 and 4) of alginate-degrading strains formerly classified as was studied. Three new group 3 and two new group 4 strains were isolated from soil and studied. High intragroup deoxyribonucleic acid (DNA) relatedness values of 87 to 96% and low intergroup vlues of 27 to 36% indicated that these groups were genetically distinct taxa. Furthermore, low DNA complementarity values of 22 to 37% established that these two groups with guanine-plus-cytosine contents of 47 to 49 mol% were not closely related genetically to the type strains of recognized species with similar base contents. Low relatedness values were also measured between strains from the two groups and , another alginate-degrading species. In general, negative reactions for growth at 50°C, for anaerobic growth, for casein hydrolysis, for nitrate reduction, and for acetylmethylcarbinol production differentiated the two groups from the named species. Growth in 0.001% lysozyme and utilization of citrate, fumarate, and succinate separated group 3 from group 4, which neither grew in lysozyme nor utilized the three organic acids. The data from the present and previous studies suggest that group 3 and 4 organisms are strains of two new species, for which are proposed the names and , respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-37-3-284
1987-07-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/37/3/ijsem-37-3-284.html?itemId=/content/journal/ijsem/10.1099/00207713-37-3-284&mimeType=html&fmt=ahah

References

  1. DeLey J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  2. Edwards P. R., Ewing W. H. 1972 Identification of Enterobacteriaceae. Burgess Publishing Co.; Minneapolis:
    [Google Scholar]
  3. Fahmy F., Flossdorf J., Claus D. 1985; The DNA base composition of the type strains of the genus Bacillus. Syst. Appl. Microbiol. 6:60–65
    [Google Scholar]
  4. Gordon R. E., Haynes W. C., Pang C. H. 1973 The genus Bacillus. Agriculture handbook 427. U.S. Department of Agriculture; Washington, D.C.:
    [Google Scholar]
  5. Hansen J. B., Scott Doubet R., Ram J. 1984; Alginase enzyme production by Bacillus circulans. Appl. Environ. Microbiol. 47:704–709
    [Google Scholar]
  6. Hansen J. B., Nakamura L. K. 1985; Distribution of alginate lyase activity among strains of Bacillus circulans. Appl. Environ. Microbiol. 49:1019–1021
    [Google Scholar]
  7. Koransky J. R., Allen S. D., Dowell V. R. Jr. 1978; Use of ethanol for selective isolation of sporeforming microorganisms. Appl. Environ. Microbiol. 35:762–765
    [Google Scholar]
  8. McDowell R. H. 1972 Properties of alginates, 3rd. Alginate Industries Limited; London:
    [Google Scholar]
  9. Nakamura L. K. 1984; Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., nom. rev., Bacillus pabuli sp. nov., nom. rev., and Bacillus validus sp. nov., nom. rev. Int. J. Syst. Bacteriol. 34:224–226
    [Google Scholar]
  10. Nakamura L. K., Swezey J. 1983; Taxonomy of Bacillus circulans Jordan 1890: base composition and reassociation of deoxyribonucleic acid. Int. J. Syst. Bacteriol. 33:46–52
    [Google Scholar]
  11. Nakamura L. K., Swezey J. 1983; Deoxyribonucleic acid relatedness of Bacillus circulans Jordan 1890 strains. Int. J. Syst. Bacteriol. 33:703–708
    [Google Scholar]
  12. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. Mol. Biol. 4:430–443
    [Google Scholar]
/content/journal/ijsem/10.1099/00207713-37-3-284
Loading
/content/journal/ijsem/10.1099/00207713-37-3-284
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error