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A new species, Rhizobium fredii, is proposed for fast-growing root nodule bacteria isolated from soybeans. The type strain was isolated from a root nodule of Glycine max growing in Honan Province, China, and is designated strain USDA 205 (= ATCC 35423 = PRC 205). This new species is differentiated from currently recognized Rhizobium and Bradyrhizobium species by deoxyribonucleic acid hybridization comparisons, plant specificity, generation times, antibiotic resistance, and serology. The strains of R. fredii are differentiated into two proposed chemovars, R. fredii chemovar fredii chemovar nov. and R. fredii chemovar siensis chemovar nov., by deoxyribonucleic acid hybridization tests, growth in the presence of erythromycin (20 μg/ml), final pH of yeast extract-mannitol medium, and serology.
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