1887

Abstract

Asaccharolytic, anaerobic vibrios which require formate and fumarate for growth in broth culture have been isolated from humans. Some of these strains resemble phenotypically, but show no deoxyribonucleic acid (DNA)-DNA homology with either or . In this investigation, six unidentified strains were compared with reference and type strains of and by using cluster analysis of phenotypic characteristics, enzyme-linked immunosorbent assays, and cell wall ultrastructure. One unidentified strain, a sewage isolate, was . The remaining unidentified strains showed no DNA-DNA homology with either or sp. nov. is proposed for four isolates which were distinct, as determined by serological and DNA-DNA homology experiments, from the previously described species. strains could also be differentiated from by morphology and by the growth of in the presence of Janus green (0.1 g/liter), basic fuchsin (0.032 g/liter), sodium deoxycholate (1.0 g/liter), indulin scarlet (0.5 g/liter), oxgall (10 g/liter), safranine (0.5 g/liter), azure II (0.025 g/liter), penicillin (16 μg/ml), and polymixin B (4 (μg/ml). and are similar in cell ultrastructure and in other phenotypic features.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-34-3-275
1984-07-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/34/3/ijs-34-3-275.html?itemId=/content/journal/ijsem/10.1099/00207713-34-3-275&mimeType=html&fmt=ahah

References

  1. Albanese E., Kafkewitz D. 1978; EflFect of medium composition on the growth and asparaginase production of Vibrio succinogenes. . Appl. Environ. Microbiol. 36:25–30
    [Google Scholar]
  2. Badger S. J., Tanner A. C. R. 1981; Serologic studies of Bacteroides gracilis, Campylobacter concisus, Wolinella recta, and Eikenella corrodens from human periodontal disease. Int. J. Syst. Bacteriol. 31:446–451
    [Google Scholar]
  3. Cowan S. T. 1974; Cowan and Steel’s manual for the identification of medical bacteria. , 2. Cambridge University Press; Cambridge:
    [Google Scholar]
  4. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J. Bacteriol. 101:738–754
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12:133–142
    [Google Scholar]
  6. Ebersole J. L., Frey D. E., Taubman M. A., Smith D. J. 1980; An ELISA for measuring serum antibodies for Actinobacillus actinomycetemcomitans. . J. Periodontal Res. 15:621–632
    [Google Scholar]
  7. Ebersole J. L., Frey D. E., Taubman M. A., Smith D. J., Socransky S. S., Tanner A. C. R. 1984; Serological identification of oral Bacteroides spp. by enzyme-linked immunosorbent assay. J. Clin. Microbiol. 19:639–644
    [Google Scholar]
  8. Kafkewitz D. 1975; Improved growth media for Vibrio succinogenes. . Appl. Microbiol. 29:121–122
    [Google Scholar]
  9. Karnovsky M. J. 1965; A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27:137A–138A
    [Google Scholar]
  10. Lai C.-H., Listgarten M. A., Tanner A. C. R., Socransky S. S. 1981; Ultrastructures of Bacteroides gracilis, Campylobacter concisus, Wolinella recta, and Eikenella corrodens, all from humans with periodontal disease. Int. J. Syst. Bacteriol. 31:465–475
    [Google Scholar]
  11. Luft J. H. 1966; Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol. 9:409–414
    [Google Scholar]
  12. Marmur M. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  13. Marmur M., Doty P. 1962; Determination of the base composition of DNA from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  14. Nakane P. K., Kawaoi A. 1974; Peroxidase-labeled antibody: a new method of conjugation. J. Histochem. Cytochem. 22:1084–1091
    [Google Scholar]
  15. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17:208–212
    [Google Scholar]
  16. Ritchie A. E., Keeler R. F., Bryner J. H. 1966; Anatomical features of Vibrio fetus. . J. Gen. Microbiol. 43:427–438
    [Google Scholar]
  17. Salanitro J. P., Muirhead P. A. 1975; Quantitative method for gas chromatographic analysis of short-chain monocarboxylic and dicarboxylic acids in fermentation media. Appl. Microbiol. 29:374–381
    [Google Scholar]
  18. Smibert R. M., Holdeman L. V. 1976; Clinical isolates of anaerobic gram-negative rods with a formate-fumarate energy metabolism: Bacteroides corrodens, Vibrio succinogenes, and unidentified strains. J. Clin. Microbiol. 3:432–437
    [Google Scholar]
  19. Sneath P. H. A., Sokal R. R. 1973; Numerical taxonomy. The principles and practice of numerical classification. W. H. Freeman and Co.; San Francisco:
    [Google Scholar]
  20. Socransky S. S., Holt S. C., Leadbetter E. R., Tanner A. C. R., Savitt E., Hammond B. F. 1979; Capnocytophaga: new genus of gram-negative gliding bacteria. III. Physiologic characterization. Arch. Microbiol. 122:29–33
    [Google Scholar]
  21. Tanner A. C. R., Badger S., Lai C.-H., Listgarten M. A., Visconti R. A., Socransky S. S. 1981; Wolinella gen. nov., Wolinella succinogenes (Vibrio succinogenes Wolin et al.) comb. nov., and description of Bacteroides gracilis sp. nov., Wolinella recta sp. nov., Campylobacter concisus sp. nov., and Eikenella corrodens from humans with periodontal disease. Int. J. Syst. Bacteriol. 31:432–445
    [Google Scholar]
  22. Tanner A. C. R., Visconti R. A., Holdeman L. V., Sundqvist G., Socransky S. S. 1982; Similarity of Wolinella recta strains isolated from periodontal pockets and root canals. J. Endodontol. 8:294–300
    [Google Scholar]
  23. Tarrand J. J., Groschel D. H. M. 1982; Rapid, modified oxidase test for oxidase-variable bacterial isolates. J. Clin. Microbiol. 16:772–774
    [Google Scholar]
  24. van Palenstein Helderman W. H., Rosman I. 1976; Hydrogen-dependent organisms from the human gingival crevice re-sembling Vibrio succinogenes. . Antonie van Leeuwenhoek J. Microbiol. Serol. 42:107–118
    [Google Scholar]
  25. Wolin M. J., Wolin E. A., Jacobs N. J. 1961; Cytochrome-producing anaerobic vibrio, Vibrio succinogenes sp. n. J. Bacteriol. 81:911–917
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-34-3-275
Loading
/content/journal/ijsem/10.1099/00207713-34-3-275
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error