1887

Abstract

Asaccharolytic, anaerobic vibrios which require formate and fumarate for growth in broth culture have been isolated from humans. Some of these strains resemble phenotypically, but show no deoxyribonucleic acid (DNA)-DNA homology with either or . In this investigation, six unidentified strains were compared with reference and type strains of and by using cluster analysis of phenotypic characteristics, enzyme-linked immunosorbent assays, and cell wall ultrastructure. One unidentified strain, a sewage isolate, was . The remaining unidentified strains showed no DNA-DNA homology with either or sp. nov. is proposed for four isolates which were distinct, as determined by serological and DNA-DNA homology experiments, from the previously described species. strains could also be differentiated from by morphology and by the growth of in the presence of Janus green (0.1 g/liter), basic fuchsin (0.032 g/liter), sodium deoxycholate (1.0 g/liter), indulin scarlet (0.5 g/liter), oxgall (10 g/liter), safranine (0.5 g/liter), azure II (0.025 g/liter), penicillin (16 μg/ml), and polymixin B (4 (μg/ml). and are similar in cell ultrastructure and in other phenotypic features.

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1984-07-01
2024-04-27
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