Analyses of 173 strains (representing 30 species) with a newly developed quantitative colorimetric , -tetramethyl--phenylenediamine oxidase assay revealed the presence of many oxidase-positive species. Of 144 mesophilic strains analyzed, the following were oxidase positive: 16 of 16 strains, 7 of 9 strains, 5 of 10 strains, 4 of 5 strains, 2 of 5 strains, 1 strain of , and 1 strain of The thermophilic organisms T-10 and ATCC 8005, “” (one strain), and “” DSM 465 were also oxidase positive. In fact, when the oxidase-positive thermophilic strains were grown at 65°C, they exhibited very high oxidation rates; most of these rates were equivalent to the high rates of reaction recorded for strains of and spp. Most mesophilic strains and all of the psychrophiles examined were oxidase negative or oxidase indeterminate (the latter had oxidase rates equilavent to the rates in the , -tetramethyl--phenylenediamine autooxidation reaction). Spectral absorbance analyses on membranes of oxidase-positive strain 385 revealed the presence of a type cytochrome with cytochrome oxidases and + ; strains which could not oxidize , -tetramethyl--phenylenediamine lacked type cytochromes but possessed these two oxidases. The paper strip Kovacs oxidase test with Marion reagent could be routinely used for detecting oxidase-positive strains. A distinct class of oxidase-positive strains should be identified, which might eventually be useful for identification studies.


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