1887

Abstract

A series of comparative studies was carried out on the Fuller and Guadalupe strains of and the vole agent described by Baker in 1946. No differences were encountered between the two strains of . . The vole agent resembled . in most of its phenotypic characteristics, but, in contrast to . , did not require CO for growth, catabolized glutamate without an added energy-yielding substrate, and autoagglutinated. Comparisons of the migration patterns of various protein fractions in gels by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, line-rocket immunoelectrophoresis, and enzyme-specific staining confirmed the identity of the two strains of . and their similarity to the vole agent. Although many of the proteins had the same function as enzymes in . and the vole agent or reacted equally well with both homologous and heterologous sera, they migrated at different rates in the gels. Therefore, on the basis of this and previously reported evidence, we propose that the vole agent be named sp. nov. This name honors J. William Vinson, who, in collaboration with Henry S. Fuller, established that . can be grown axenically and initiated studies of the genus by conventional bacteriological techniques. The type strain of . is ATCC VR-152.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-32-3-305
1982-07-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/32/3/ijs-32-3-305.html?itemId=/content/journal/ijsem/10.1099/00207713-32-3-305&mimeType=html&fmt=ahah

References

  1. Axelsen N. H., Krøll J., Weeke B. 1973; A manual of quantitative immunoelectrophoresis: methods and applications. Scand. J. Immunol 2: Suppl. 1 1–169
    [Google Scholar]
  2. Baker J. A. 1946; A rickettsial infection in Canadian voles. J. Exp. Med 84:37–51
    [Google Scholar]
  3. Beauchamp C. O., Fridovich I. 1971; Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem 44:276–287
    [Google Scholar]
  4. Bozeman F. M., Shirai A., Humphries J. W., Fuller H. S. 1967; Ecology of Rocky Mountain spotted fever. II. Natural infection of wild mammals and birds in Virginia and Maryland. Am. J. Trop. Med. Hyg 16:48–59
    [Google Scholar]
  5. Brezina R. 1978; Phase variation phenomenon in Coxiella bumetii. 221–235 Kazár J., Ormsbee R. A., Tarasevich I. N. Rickettsiae and rickettsial diseases VEDA; Bratislava, Czechoslovakia:
    [Google Scholar]
  6. Codeleoncini E. 1946; Sulla presenza in Etiopia della Rickettsia weigli. Boll. Soc. Ital. Med. Igiene Trop. Sezione Eritrea 6:129–151
    [Google Scholar]
  7. Coolbaugh J. C., Progar J. J., Weiss E. 1976; Enzymatic activities of cell-free extracts of Rickettsia typhi. Infect. Immun 14:298–305
    [Google Scholar]
  8. Dasch G. A., Samms J. R., Weiss E. 1978; Biochemical characteristics of typhus group rickettsiae with special attention to the Rickettsia prowazekii strains isolated from flying squirrels. Infect. Immun 19:676–685
    [Google Scholar]
  9. Dasch G. A., Weiss E. 1977; Characterization of the Madrid E strain of Rickettsia prowazekii purified by Renografin density gradient centrifugation. Infect. Immun 15:280–286
    [Google Scholar]
  10. Huang K.-Y. 1967; Metabolic activity of the trench fever rickettsia, Rickettsia quintana. J. Bacteriol 93:853–859
    [Google Scholar]
  11. Ito S., Vinson J.W. 1965; Fine structure of Rickettsia quintana cultivated in vitro and in the louse. J. Bacteriol 80:481–495
    [Google Scholar]
  12. Jackson E. B., Danauskas J. X., Coale M. C., Smadel J.E. 1957; Recovery of Rickettsia akari from the Korean vole, Microtus fortis pelliceus. Am. J. Hyg 66:301–308
    [Google Scholar]
  13. Kopmans-Gargantiel A. I., Wisseman C. L. Jr 1981; Differential requirements for enriched atmospheric carbon dioxide content for intracellular growth in cell culture among selected members of the genus Rickettsia. Infect. Immun 31:1277–1280
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R.J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chem 193:265–275
    [Google Scholar]
  16. Mason R. A. 1970; Propagation and growth of Rickettsia quintana in a new liquid medium. J. Bacteriol 103:184–190
    [Google Scholar]
  17. Merrell B. R., Weiss E., Dasch G.A. 1978; Morphological and cell association characteristics of Rochali-maea quintana: comparison of the vole and Fuller strains. J. Bacteriol 135:633–640
    [Google Scholar]
  18. Mooser H., Leemann A., Chao S. H., Gubler H.U. 1948; Beobachtungen an Funftagefieber. Schweiz. Z. Allg. Pathol., Bakteriol 11:513–522
    [Google Scholar]
  19. Myers W. F., Cutler L. D., Wisseman C. L. Jr 1969; Role of erythrocytes and serum in the nutrition of Rickettsia quintana. J. Bacteriol 97:663–666
    [Google Scholar]
  20. Myers W. F., Osterman J. V., Wisseman C. L. Jr 1972; Nutritional studies of Rickettsia quintana: nature of the hematin requirement. J. Bacteriol 109:89–95
    [Google Scholar]
  21. Myers W. F., Warfel L. E., Wisseman C. L. Jr 1978; Absence of hydrogen peroxide production by or catalase action in Rickettsia prowazeki. J. Bacteriol 136:452–454
    [Google Scholar]
  22. Myers W. F., Wisseman C. L. Jr, Fiset P., Oaks E. V., Smith J.F. 1979; Taxonomic relationship of vole agent to Rochalimaea quintana. Infect. Immun 26:976–983
    [Google Scholar]
  23. Pshenichnov A. V. 1959; Results of many years’ combined study of trench fever. J. Microbiol. Epidemiol. Immunobiol USSR: 281392–1397
    [Google Scholar]
  24. Pshenichnov A. V., Pshenichnov R. A., Pecherkina A. A., Plaksina A.N. 1965 Cultivation of pathogenic rickettsiae in cell-free semisynthetic nutrient mediumFed. Proc. Transl Suppl 24:T31–T33
    [Google Scholar]
  25. Rutherford W. J. 1916; Trench fever: the field vole a possible origin. Br. Med. J 2:386–387
    [Google Scholar]
  26. Sikora H. 1921; Uber die Zuchtung der Rickettsia pedi-culi. Arch. Schiffs Trop. Hyg 25:123–124
    [Google Scholar]
  27. Sparrow H. 1961; Sur une souche de Rickettsia quintana isolée en Tunisie. Pathol. Microbiol 24: Suppl 140–148
    [Google Scholar]
  28. Strong R. P. 1918; Trench fever. Report of Commission, Medical Research Committee, American Red Cross Oxford University Press; Oxford:
    [Google Scholar]
  29. Tyeryar F. J. Jr, Weiss E., Millar D. B., Bozeman F. M., Ormsbee R.A. 1973; DNA base composition of rickettsiae. Science 180:415–417
    [Google Scholar]
  30. Varela G., Fournier R., Mooser H. 1954; Presencia de Rickettsia quintana en piojos Pediculus humanus de la Ciudad de Mexico. Inoculation experimental. Rev. Inst. Salubr. Enferm. Trop Mexico City: 1439–42
    [Google Scholar]
  31. Varela G., Vinson J. W., Molina-Pasquel C. 1969; Trench fever. II. Propagation of Rickettsia quintana on cell-free medium from the blood of two patients. Am. J. Trop. Med. Hyg 18:708–712
    [Google Scholar]
  32. Vinson J. W. 1964; Etiology of trench fever in Mexico. Ind. Trop. Health 5:109–114
    [Google Scholar]
  33. Vinson J. W. 1966; In vitro cultivation of the rickettsial agent of trench fever. Bull. W.H.O 35:155–164
    [Google Scholar]
  34. Vinson J. W., Fuller H.S. 1961; Studies on trench fever. I. Propagation of rickettsia-like microorganisms from a patient’s blood. Pathol. Microbiol 24: Suppl 152–166
    [Google Scholar]
  35. Vinson J. W., Varela G., Molina-Pasquel C. 1969; Trench fever. III. Induction of clinical disease in volunteers inoculated with R.quintana propagated on blood agar. Am. J. Trop. Med. Hyg 18:713–722
    [Google Scholar]
  36. Weiss E. 1970; Catabolic activities oiNeisseria meningiti-dis: utilization of succinate. J. Bacteriol 101:133–137
    [Google Scholar]
  37. Weiss E. 1973; Growth and physiology of rickettsiae. Bacteriol. Rev 27:259–283
    [Google Scholar]
  38. Weiss E. 1981; Biochemistry and metabolism of rickettsiae: current trends. 387–400 Burgdorfer W., Anacker R. L. Rickettsiae and rickettsial diseases Academic Press, Inc; New York:
    [Google Scholar]
  39. Weiss E., Dasch G. A., Woodman D. R., Williams J.C. 1978; Vole agent identified as a strain of the trench fever rickettsia, Rochalimaea quintana. Infect. Immun 19:1013–1020
    [Google Scholar]
  40. Weiss E., Mamay H. K., Dasch G.A. 1982; Orni-thine metabolism in the genus Rochalimaea. J. Bacteriol 150:245–250
    [Google Scholar]
  41. Weiss E., Moulder J.W. 1974; Genus II. Rochalimaea (Macchiavello) Krieg 1961, 162. 890–891 Buchanan R. E., Gibbons N. E. Bergey’s manual of determinative bacteriology, 8. The Williams & Wilkins Co; Baltimore:
    [Google Scholar]
  42. Weiss E., Peacock M. G., Williams J.C. 1980; Glucose and glutamate metabolism of Legionella pneumo-phila. Curr. Microbiol 4:1–6
    [Google Scholar]
  43. Weiss E., Rees H. B. Jr, Hayes J.R. 1967; Metabolic activity of purified suspensions of Rickettsia rick-ettsi. Nature London: 2131020–1022
    [Google Scholar]
  44. Wisseman C. L. Jr, Hahn F. E., Jackson E. B., Bozeman F. M., Smadel J.E. 1952; Metabolic studies of rickettsiae. II. Studies on the pathway of glutamate oxidation by purified suspensions of Rickettsia mooseri. J. Immunol 68:251–264
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-32-3-305
Loading
/content/journal/ijsem/10.1099/00207713-32-3-305
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error