We used polyacrylamide slab gel electrophoresis with specific staining for seven enzymes to compare 108 strains belonging to the genera and The patterns obtained allowed us to divide the strains of and its varieties into six groups. The strains of and examined had identical patterns. var. and var. clearly differed from and in their hexokinase and 6-phosphogluconate dehydrogenase patterns. , and each had a characteristic pattern. We found three haploid mating type strains to mating type a of , mating type a of , and mating type α of among the strains of var. var. , and var. by performing conjugation tests to confirm the relationships that were revealed when the enzyme patterns were compared. In the genus , compatible mating type strains showed similar enzymatic patterns. Strains of which could not assimilate potassium nitrate had the same enzymatic patterns as those that could.


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