1887

Abstract

Abstract

A total of 50 staphylococcal strains of animal origin were identified as members of Hájek on the basis that they possessed all of the characteristics originally reported by Hájek for except one, acetoin production. The Barritt method failed to demonstrate acetoin production by these isolates, whereas in the case of a micromethod in which pyruvate was used as substrate, acetoin production was detected. We discuss both the discrepancy observed between the results of the two methods and the taxonomic implications of our findings.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-31-3-232
1981-07-01
2022-08-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/31/3/ijs-31-3-232.html?itemId=/content/journal/ijsem/10.1099/00207713-31-3-232&mimeType=html&fmt=ahah

References

  1. Abd-el-Malek Y., Gibson T. 1948; Studies in the bacteriology of milk. II. The staphylococci and micrococci of milk. J. Dairy Res. 15:249–260
    [Google Scholar]
  2. Baird-Parker A. C. 1966; Methods for classifying staphylococci and micrococci. 59–64 In Gibbs B. M., Skinner F. A. (ed.) Identification methods for microbiologists, part A. Academic Press, Inc.; New York:
    [Google Scholar]
  3. Baird-Parker A. C. 1974; Micrococcaceae. 478–490 In Buchanan R. E., Gibbons N. E. (ed.) Bergey's manual of determinative bacteriology, 8th ed.. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  4. Barritt M. M. 1936; The intensification of the Voges-Proskauer reaction by the addition of a-naphthol. J. Pathol. Bacteriol. 42:441–454
    [Google Scholar]
  5. Brun Y., Fleurette J., Forey F. 1978; Micro-method for biochemical identification of coagulase-negative staphylococci. J. Clin. Microbiol. 8:503–508
    [Google Scholar]
  6. Davis G. H. G., Hoyling B. 1973; Use of a rapid acetoin test in the identification of staphylococci and micrococci. Int. J. Syst. Bacteriol. 23:281–282
    [Google Scholar]
  7. Eddy B. P. 1961; The Voges Proskauer reaction and its significance: a review. J. Appl. Bacteriol. 24:27–41
    [Google Scholar]
  8. Flandrois J. P., Fleurette J., Eyraud F. 1975; Detection de la protéine A de Staphylococcus aureus par hemagglutination conditionnée. Ann. Biol. Clin. 33:365–368
    [Google Scholar]
  9. Hájek V. 1976; Staphylococcus intermedius, a new species isolated from animals. Int. J. Syst. Bacteriol. 26:401–408
    [Google Scholar]
  10. Hájek V., Maršálek E. 1971; The differentiation of pathogenic staphylococci and a suggestion for their taxonomic classification. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 217:176–182
    [Google Scholar]
  11. Hájek V., Maršálek E. 1976; Evaluation of classificatory criteria for staphylococci. 11–21 In Jeljasjewicz J. (ed.) Staphylococci and staphylococcal diseases Gustav Fisher Verlag; Stuttgart:
    [Google Scholar]
  12. Happold F. C., Spencer C. P. 1952; The enzymatic formation of acetyl-methylcarbinol and related compounds. Biochim. Biophys. Acta. 8:543
    [Google Scholar]
  13. Kocur M. 1973; The taxonomy of the genus Staphylococcus. 5–12 In Jeljasjewicz J. (ed.) Staphylococci and staphylococcal infections Polish Medical Publishers; Warsaw:
    [Google Scholar]
  14. Oeding P., Haukenes G. 1963; Identification of Staphylococcus aureus antigens and antibodies by means of the gel precipitation technique. Acta Pathol. Microbiol. Scand. 57:438–450
    [Google Scholar]
  15. Páčová Z., Kocur M. 1973; A rapid microtest for the detection of acetoin. Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 1 Orig. Reihe A 223:106–110
    [Google Scholar]
  16. Segal B. 1940; The utilization of acetyl methylcarbinol by Staphylococcus albus and S. aureus. J. Bacteriol. 39:747–749
    [Google Scholar]
  17. Shimizu A., Kato E. 1979; Bacteriophage typing of Staphylococcus aureus isolated from dogs in Japan. Jpn. J. Vet. Sci. 41:405–408
    [Google Scholar]
  18. Strecker H. J., Harary I. 1954; Bacterial butylene glycol deshydrogenase and diacetyl reductase. J. Biol. Chem. 211:263–270
    [Google Scholar]
  19. Tissler R. P. 1938; The fermentation of acetyl methylcarbin by the Escherichia, Aerobacter group and its significance in the Voges-Proskauer reaction. J. Bacteriol. 35:157–162
    [Google Scholar]
  20. Watt D., Werkman C. H. 1951; Modification of the enzyme system of Micrococcus pyogenes. Arch. Bio-chem. 31:383–390
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-31-3-232
Loading
/content/journal/ijsem/10.1099/00207713-31-3-232
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error