1887

Abstract

Analysis of representative strains of the eight human serotypes of by polyacrylamide gel electrophoresis identified 36 to 40 polypeptides for each strain. At least 80% of the peptides were common among strains, but unique major peptides were identifiable in ureaplasmic types. Type 1 had a polypeptide of 85,000 daltons, type 3 had a polypeptide of 72,000 daltons, type 5 had a polypeptide of 64,000 daltons, and type 8 had a polypeptide of 95,000 daltons. The unique polypeptides in types 1 and 8 were identified as membrane components. Two common major components of 44,000 and 70,000 daltons were observed. Several components were common to some, but not all, serotypes. Patterns obtained from strains were strikingly different from the patterns of and Isoelectric focusing demonstrated a unique membrane protein for type 1 at pK 6.4, whereas type 8 possessed an assembly of five unique proteins at pK 7.0. Ureaplasmata were strongly similar to each other by isoelectric focusing, but strikingly different from members of the other genera studied. Although a filtered, strongly buffered dialysate medium with 1% serum and 30 mM urea was used both to maximize yields and to minimize contamination, minor contaminants were detected, which comigrated with horse transferrin (pK 6.0) and cytochrome c (molecular weight, 14,000). The similarities of the polypeptide patterns of strains affirm their close relationships to each other, in contrast to the diversity shown in the genus and our recognition of type-specific membrane peptides will enhance the identification of serotypes and the classification of strains.

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1980-01-01
2024-10-12
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References

  1. Bagdasasian M., Matheson N. A., Synge R. L. M., Youngson M. A. 1964; New procedures for isolating polypeptides and proteins from tissues. Biochem. J 91:91–105
    [Google Scholar]
  2. Bradbury J. M., Jordan F. T. W. 1972; Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination reaction. J. Hyg 70:267–278
    [Google Scholar]
  3. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis viruses. J. Exp. Med 99:167–182
    [Google Scholar]
  4. Goel M. C. 1973; New method for the isolation of mem branes from Mycoplasma gallisepticum. . J. Bacteriol 116:994–1000
    [Google Scholar]
  5. Kenny G. E. 1967; Heat-lability and organic solvent- solubility of Mycoplasma antigens. Ann. N.Y. Acad. Sci 143:676–681
    [Google Scholar]
  6. Kenny G. E. 1969; Serological comparison of ten glyco lytic Mycoplasma species. J. Bacteriol 98:1044–1055
    [Google Scholar]
  7. Kenny G. E. 1979; Antigenic determinants. p 351–384 In Barile M. F., Razin S. ed The mycoplasmas. vol 1: Academic Press Inc; New York:
    [Google Scholar]
  8. Kenny G. E., Cartwright F. D. 1977; Effect of urea concentration on growth of Ureaplasma urealyticum (T-strain mycoplasma). J. Bacteriol 132:144–150
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. Biol. Chem 193:265–275
    [Google Scholar]
  11. Masover G. K., Razin S., Hayflick L. 1977; Lo calization of enzymes in Ureaplasma urealyticum (T- strain mycoplasma). J. Bacteriol 130:297–302
    [Google Scholar]
  12. Pollack J. D. 1979; Respiratory pathways and energy- yielding mechanisms. p 187–211 In Barile M. F., Razin S. ed The mycoplasmas. vol 1 Academic Press Inc; New York:
    [Google Scholar]
  13. Razin S. 1978; The mycoplasmas. Microbiol. Rev 42:414–470
    [Google Scholar]
  14. Razin S., Argaman M. 1963; Lysis of mycoplasma, bacterial protoplasts, spheroplasts and L-forms by var-ious agents. J. Gen. Microbiol 30:155–172
    [Google Scholar]
  15. Razin S., Valdesuso J., Purcell R. H., Chanock R. M. 1970; Electrophoretic analysis of cell proteins of T-strain mycoplasmas isolated from man. J. Bacteriol 103:702–706
    [Google Scholar]
  16. Romano N., LaLicata R. 1978; Cell fractionation and enzymatic activities of Ureaplasma urealyticum. . J. Bacteriol 136:833–838
    [Google Scholar]
  17. Rottem S., Razin S. 1967; Electrophoretic patterns of membrane proteins of Mycoplasma. . J. Bacteriol 94:359–364
    [Google Scholar]
  18. Rottem S., Razin S. 1972; Isolation of mycoplasma membranes by digitonin. J. Bacteriol 110:699–705
    [Google Scholar]
  19. Sayed I. A., Hatten B. A. 1976; Isoelectric focusing of mycoplasma proteins. Appl. Environ. Microbiol 32:603–609
    [Google Scholar]
  20. Sayed I. A., Kenny G. E. 1978; Effect of ammo nium ion concentration and osmotic pressure on growth of Ureaplasma urealyticum (T-strain mycoplasma). J. Bacteriol 134:962–967
    [Google Scholar]
  21. Sayed I. A., Kenny G. E. 1979; Effects of reducing agents, catalase, and medium reuse on toxicity of media for growth of Ureaplasma urealyticum. . J. Infect. Dis 139:720–724
    [Google Scholar]
  22. Shapiro A. L., Vinuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem. Biophys. Res. Commun 28:815–820
    [Google Scholar]
  23. Shepard M. C., Lunceford C. D. 1978; Serological typing of Ureaplasma urealyticum isolates from urethritis patients by an agar growth inhibition method. J. Clin. Microbiol 8:566–574
    [Google Scholar]
  24. Shepard M. C., Lunceford C. D., Ford D. K., Purcell R. H., Taylor-Robinson D., Razin S., Black F. T. 1974; Ureaplasma urealyticum gen. nov., sp. nov.: proposed nomenclature for the human T (T- strain) mycoplasmas. Int. J. Syst. Bacteriol 24:160–171
    [Google Scholar]
  25. Thirkill C. E., Kenny G. E. 1974; Serological comparison of five arginine-utilizing Mycoplasma species by two-dimensional immunoelectrophoresis. Infect. Immun 10:624–632
    [Google Scholar]
  26. Vesterberg O. 1973; Isoelectric focusing of proteins in thin layers of polyacrylamide gel. Sci. Tools 20:22–29
    [Google Scholar]
  27. Weber K., Osborn M. 1969; The reliability of molecular weight determinatiions by dodecyl sulfate- polyacrylamide gel electrophoresis. J. Biol. Chem 244:4406–4412
    [Google Scholar]
  28. Yaguzhinskaya O. E. 1976; Detection of serum proteins in the electrophoretic patterns of total proteins of my-coplasma cells. J. Hyg 77:189–198
    [Google Scholar]
  29. Zola H., Baxendale W., Sayer L. J. 1970; Poly acrylamide gel electrophoresis of lysates of mycoplas-mas. Res. Vet. Sci 11:397–399
    [Google Scholar]
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