Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 29, Issue 4

Rapid Test for Determining the Intracellullar Rhodanese Activity of Various Bacteria Free

Abstract

A simple, reproducible technique with potential taxonomic application was developed for the rapid detection of rhodanese activity in gram-negative and gram-positive bacteria. The method requires suspension of the growth from three colonies in a solution of lysozyme and ethylenediaminetetraacetic acid for 60 min. After cell lysis, the presence of rhodanese activity is determined colorimetrically by measuring the amount of thiocyanate formed from thiosulfate and potassium cyanide by use of ferric nitrate. By this technique, a survey of 411 bacterial strains revealed the presence of rhodanese in all test strains of , and . No activity was detected in , or species. Randomly selected strains which did not exhibit rhodanese activity were confirmed to be rhodanese-negative by assay of mechanically disrupted cells harvested from 500 ml of growth medium.

  • Published Online:
© Society for General Microbiology, 1979
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-29-4-339
1979-10-01
2025-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/29/4/ijs-29-4-339.html?itemId=/content/journal/ijsem/10.1099/00207713-29-4-339&mimeType=html&fmt=ahah

References

  1. Hall M. R., Berk R. S. 1968; Microbial growth on mercaptosuccinic acid. Can. J. Microbiol 14:515–523
     [Google Scholar]
  2. Kaback H. R. 1971; Bacterial membranes. Methods En- zymol 22:99–120
     [Google Scholar]
  3. Koch A. L., Putnam S. L. 1971; Sensitive biuret method for determination of protein in an impure sys-tem such as whole bacteria. Anal. Biochem 44:239–245
     [Google Scholar]
  4. Lang K. 1933; Die Rhodanbildung in Tierkorper. Bio chem. Z 259:243–256
     [Google Scholar]
  5. Møiler V. 1954; Diagnostic use of the Braun KCN test within the enterobacteriaceae. Acta Pathol. Microbiol. Scand 34:115–126
     [Google Scholar]
  6. Schook L. B., Berk R. S. 1978; Nutritional studies with Pseudomonas aeruginosa grown on inorganic sul-fur sources. J. Bacteriol 133:1377–1382
     [Google Scholar]
  7. Sörbo B. H. 1953; Crystalline rhodanese. Acta Chem. Scand 7:1129–1136
     [Google Scholar]
  8. Sorbo B. H. 1960; Rhodanese. Enzymology 2:334–337
     [Google Scholar]
  9. Toru K., Bandurski R. S. 1967; Yeast sulfatereducing system. Biochim. Biophys. Acta 136:286–295
     [Google Scholar]
  10. Villarejo M., Westley J. 1963; Rhodanese-cata-lyzed reduction of thiosulfate by reduced lipoic acid. J. Biol. Chem 238:1185–1186
     [Google Scholar]
  11. Yoch D. C., Lindstrom E. S. 1971; Survey of photosynthetic bacteria for rhodanese (thiosulfate: cyanide sulfur transferase) activity. J. Bacteriol 106:700–701
     [Google Scholar]
/content/journal/ijsem/10.1099/00207713-29-4-339
Loading
Rapid Test for Determining the Intracellullar Rhodanese Activity of Various Bacteria
Int J Syst Evol Microbiol 29, 339 (1979); https://doi.org/10.1099/00207713-29-4-339
/content/journal/ijsem/10.1099/00207713-29-4-339
/content/journal/ijsem/10.1099/00207713-29-4-339
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error