Polynucleotide sequence relationships among strains of were assessed by analysis of deoxyribonucleic acid-deoxyribonucleic acid homo and heteroduplexes with endonuclease S1. The results showed that strains isolated from different subjects form a very tight group, with deoxyribonucleic acid homology levels ranging between 93 and 100%. Physiological tests of 23 strains included 30 different substrates. Results were remarkably uniform. All 23 strains grew better anaerobically with added H and CO than aerobically. However, colony size was greater on blood agar but not Trypticase soy (BBL Microbiology Systems, Cockeysville, Md.)-yeast extract agar with an atmosphere of 4% O with added H and CO than with anaerobic incubation. The data suggest that these strains are sufficiently closely related to justify their inclusion in a single discrete species, but their appropriate generic classification remains to be resolved.


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