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The genetic relatedness among streptomycetes capable of producing tetracycline, chlortetracycline, and oxytetracycline was studied by using deoxyribonucleic acid (DNA) association techniques. Reciprocal DNA association experiments yielded genetic similarity values ranging from 75 to 97% among Streptomyces aureofaciens ATCC 10762, S. aureofaciens ATCC 13911, S. lusitanus CBS 101A, and S. lusitanus var. tetracyclini 106-T ATCC 15843. These four strains should thus be considered members of the same species on the basis of genetic relatedness. On the bases of DNA association experiments, the degree of nucleotide similarity of morphologically heterogeneous strains with S. rimosus ATCC-10970 was 28 to 33%.
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