Nine strains of leptospires representing six serotypes were dried in vacuo from the liquid state and stored at 4 C. Viable organisms were recovered from all dried cultures during and after 10 years of storage. Motile forms were not observed immediately after rehydration, but they became apparent after several days of incubation.


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  1. Alexander A. D., Lessel E. F., Evans L. B., Franck E., Green S. S. 1972 Preservation of leptospiras by liquid-nitrogen refrigeration. Int. J. Syst. Bacteriol. 22:165–169
    [Google Scholar]
  2. Annear D. I. 1956 Preservation of leptospirae by drying. J. Pathol. Bacteriol. 72:322–323
    [Google Scholar]
  3. Annear D. I. 1962 Preservation of leptospires by drying from the liquid state. J. Gen. Microbiol. 27:341–343
    [Google Scholar]
  4. Annear D. I. 1962 Recoveries of bacteria after drying on cellulose fibres. Aust. J. Exp. Biol. Med. Sci 40:1–8
    [Google Scholar]
  5. Annear D. I. 1964 Recoveries of bacteria after drying in glutamate and other substances. Aust. J. Exp. Biol. Med. Sci. 42:717–722
    [Google Scholar]
  6. Annear D. I. 1965; Recoveries of Strigomonas oncopelti, leptospirae and Reiter’s treponeme from desiccates after storage for five years. Aust. J. Exp. Biol. Med. Sci. 43:683–684
    [Google Scholar]
  7. Cruickshank R. (ed.). 1965 Medical microbiology. E. & S. Livingstone, Ltd.; Edinburgh:
  8. Otsuka S., Manoko K. 1961 Studies on the preservation of leptospirae by freeze-drying. Jap. J. Microbiol. 5:141–148
    [Google Scholar]

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