1887

Abstract

Micrococci were commonly isolated from the skins of people living in various regions of the United States. Not all micrococci isolated in this investigation could be identified with the currently recognized species of , viz., , or , and these micrococci therefore became the subject of further taxonomic study. As a result of this study, two new species are proposed: and . The type strains of these species are ATCC 27566 and ATCC 27570, respectively. Numerous strains were isolated that were similar to or , species that were previously represented by only single strains. (ZoBell’s strain 541 [ATCC 14392; CCM 314] is designated herein as the type strain of .) A few micrococci were left unclassified. A variety of morphological, physiological, biochemical, and genetic characters were examined for their use as taxonomic criteria, and key characters, many of which can be determined by simple laboratory procedures, were selected for species differentiation. The more sophisticated studies of aliphatic hydrocarbons and cell-wall peptidoglycans were also very useful in the taxonomy of the micrococci. The predominant micrococci found on human skin were and .

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1974-01-01
2022-01-18
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References

  1. Bailey W. R., Scott E. G. 1966 Diagnostic microbiology. The C. V. Mosby Co.; St. Louis:
    [Google Scholar]
  2. Baird-Parker A. C. 1965; The classification of staphylococci and micrococci from world-wide sources.. J. Gen. Microbiol. 38:363–387
    [Google Scholar]
  3. Barksdale L. 1970; Corynebacterium diphtheriae and its relatives. Bacteriol. Rev. 34:378–422
    [Google Scholar]
  4. Bogdanovsky D., Interschick-Niebler K. H., Schleifer F. Fedler., Kandler O. 1971; 7-Glutamyl-glutamic acid an interpeptide bridge in the murein of some Micrococci and Arthro- bacter sp.. Eur. J. Biochem. 22:173–178
    [Google Scholar]
  5. Bohacek J., Kocur M., Martinec T. 1970; DNA base composition of some Micrococcaceae. Microbios 6:85–91
    [Google Scholar]
  6. Breed R. S., Murray E. G. D., Smith N. R. 1957 Bergey’s manual of determinative bacteriology, 7th ed. The Williams & Wilkins Co.; Baltimore:
    [Google Scholar]
  7. Coblentz L. M. 1943; Rapid detection of the production of acetylmethylcarbinol. Amer. J. Pub. Health 33:815–817
    [Google Scholar]
  8. Conn H. J., Dimmick I. 1947; Soil bacteria similar in morphology to Mycobacterium and Corynebacterium.. J. Bacteriol 54:291–303
    [Google Scholar]
  9. Cowan S. T., Steel K. J. 1965 Manual for the identification of medical bacteria. Cambridge University Press;
    [Google Scholar]
  10. Cziharz B., Schleifer K. H., Kandler O. A new type of peptide subunit in the murein of Arthrobacter strain J39. Biochemistry 10:3574–3578
    [Google Scholar]
  11. Deibel R. H., Evans J. B. 1960; Modified benzidine test for the detection of cytochrome- containing respiratory systems in microorganisms. J. Bacteriol 79:356–360
    [Google Scholar]
  12. Eisenberg R. C., Evans J. B. 1963; Energy and nitrogen requirements of Micrococcus roseus.. Can. J. Microbiol 9:633–642
    [Google Scholar]
  13. Evans J. B., Kloos W. E. 1972; Use of shake cultures in a semisolid thioglycolate medium for differentiating staphylococci from micrococci. Appl. Microbiol 23:326–331
    [Google Scholar]
  14. Glass M. 1973; Sarcina species on the skin of the human forearm. Trans. St. John’s Hosp. Dermatol. Soc 59:56–60
    [Google Scholar]
  15. Goodfellow M., Fleming A., Sackin M. J. Numerical classification of “Mycobacterium” rhodochrous and Runyon’s Group IV mycobacteria. Int. J. Sy st. Bacteriol 22:81–98
    [Google Scholar]
  16. Kandler O., Schleifer K. H., Dandl R. 1968; Differentiation of Streptococcus faecalis An- drewes and Horder and Streptococcus faecium Orla-Jensen based on the amino acid composition of their murein.. J. Bacteriol 96:1935–1939
    [Google Scholar]
  17. Klesius P. H., Schuhardt V. T. 1968; Use of lysostaphin in the isolation of highly polymerized deoxyribonucleic acid and in the taxonomy of aerobic Micrococcaceae.. J. Bacteriol 95:739–743
    [Google Scholar]
  18. Kligman A. M. 1965 The bacteriology of normal skin p.13-31. In Maibach H. I., Hildick-Smith G. (ed.) Skin bacteria and their role in infection McGraw-Hill Book Co.; New York:
    [Google Scholar]
  19. Kloos W. E. 1969; Transformation of Micrococcus lysodeikticus by various members of the family Micrococcaceae.. J. Gen. Microbiol 59:247–255
    [Google Scholar]
  20. Kloos W. E., Rose N. E. 1970; Transformation mapping of tryptophan loci in Micrococcus luteus. . Genetics 66:595–605
    [Google Scholar]
  21. Kocur M., Martinec T. 1965; Some remarks on the classification of micrococci.. Int. Bull. Bacteriol. Nomenci. Taxon. 15:113–114
    [Google Scholar]
  22. Kocur M., Martinec T. 1972; Taxonomic status of Micrococcus varians Migula 1900 and designation of the neotype strain.. Int. J. Syst. Bacteriol 22:228–232
    [Google Scholar]
  23. Kocur M., Pácová Z. 1970; The taxonomic status of Micrococcus roseus Flügge, 1886. Int. J. Syst. Bacteriol 20:233–240
    [Google Scholar]
  24. Kocur M., Pácová Z., Martinec T. 1972; Taxonomic status of Micrococcus luteus (Schroeter 1872) Cohn 1872, and designation of the neotype strain.. Int. J. Syst. Bacteriol. 22:218–223
    [Google Scholar]
  25. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature (London) 178:703
    [Google Scholar]
  26. Lachica R. V. F., Hoeprich P. D., Genigeorgis C. Nuclease production and lysostaphin susceptibility of Staphylococcus aureus and other catalase-positive cocci. Appl.. Microbiol 21:823–826
    [Google Scholar]
  27. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 4:109–118
    [Google Scholar]
  28. Marples M. J. 1965 The ecology of the human skin.. Charles C Thomas, Publishers, Springfield.;
    [Google Scholar]
  29. Marples R. R. 1965 The effect of hydration on the bacterial flora of the skin p.33-47. In Maibach H. I., Hildick-Smith G. (ed.) Skin bacteria and their role in infection McGraw-Hill Book Co.; New York:
    [Google Scholar]
  30. Marples R. R., Kligman A. M., Lands L. R., Downing D. T. 1970; The role of the aerobic microflora in the genesis of fatty acids in human surface lipids.. J. Invest. Dermatol 55:173–178
    [Google Scholar]
  31. Morrison S. J., Tornabene T. G., Kloos W. E. 1971; Neutral lipids in the study of relationships of members of the family Micrococcaceae.. J. Bacteriol 108:353–358
    [Google Scholar]
  32. Naylor H. B., Burgi E. 1956; Observations on abortive infection of Micrococcus lysodeikti- cus with bacteriophage.. Virology 2:577–593
    [Google Scholar]
  33. Noble W. C. 1969; Skin carriage of the Micrococcaceae. . J. Clin. Pathol 22:249–253
    [Google Scholar]
  34. Onishi H., Kamekura M. 1972; Micrococcus halobius sp. n.. Int. J. Syst. Bacteriol. 22:233–236
    [Google Scholar]
  35. Oro J., Tornabene T. G., Nooner D. W., Gelpi E. 1967; Aliphatic hydrocarbons and fatty acids of some marine and freshwater microorganisms.. J. Bacteriol 93:1811–1818
    [Google Scholar]
  36. Rosypal S., Rosypalová A., Horejs J. 1966; The classification of micrococci and staphylococci based on their DNA base composition and Adansonian analysis.. J. Gen. Microbiol. 4:281–292
    [Google Scholar]
  37. Schleifer K. H., Kandler O. 1967; Zur chemischen Zusammensetzung der Zellwand der Streptokokken.. I. Die Aminosäuresequenz des Mureins von Str. thermophilus und Str. faecalis. Arch. Mikrobiol 57:335–367
    [Google Scholar]
  38. Schleifer K. H., Kandler O. 1970; Amino acid sequence of the murein of Planococcus and other Micrococcaceae.. J. Bacteriol 103:387–392
    [Google Scholar]
  39. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol. Rev. 36:407–477
    [Google Scholar]
  40. Schleifer K. H., Kloos W. E., Moore A. Taxonomic status of Micrococcus luteus (Schroeter 1872) Cohn 1872: correlation between peptidoglycan type and genetic compatibility.. Int. J. Syst. Bacteriol 22:224–227
    [Google Scholar]
  41. Subcommittee on Taxonomy of Staphylococci and Micrococci 1965; Recommendations. Int. Bull. Bacteriol. Nomenci. Taxon. 15:109–110
    [Google Scholar]
  42. Tornabene T. G., Gelpi E., Oró J. 1967; Identification of fatty acids and aliphatic hydrocarbons in Sarcina lutea by gas chromatography and combined gas chromatography-mass spectrometry.. J. Bacteriol 94:333–343
    [Google Scholar]
  43. Tornabene T. G., Markey S. P. 1971; Characterization of branched monounsaturated hydrocarbons of Sarcina lutea and Sarcina flava. Lipids 6:190–195
    [Google Scholar]
  44. Tornabene T. G., Morrison S. J., Kloos W. E. 1970; Aliphatic hydrocarbon contents of various members of the family Micrococcaceae.. Lipids 5:929–937
    [Google Scholar]
  45. Williamson P. 1965 Quantitative estimation of cutaneous bacteria p.3-11. In Maibach H. I., Hildick-Smith G. (ed.) Skin bacteria and their role in infection McGraw-Hill Book Co.; New York:
    [Google Scholar]
  46. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria. III. DNA base composition of coryneform bacteria.. J. Gen. Appl. Microbiol 16:215–224
    [Google Scholar]
  47. ZoBell C. E., Upham C. 1944; A list of marine bacteria including descriptions of sixty new species.. Bull. Scripps Inst. Oceanogr. Univ. Calif. 5:239–292
    [Google Scholar]
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