1887

Abstract

Fifteen strains, including some belonging to , or , incorporated radioactivity into their lipids when grown in a medium containing [1-C] acetate. The majority of the radioactivity was found in the glycolipid (43%) and the polar lipid (55%) fractions. The amount of radioactivity incorporated into the lipids (disintegrations per minute per milligram of lipid) of strains of 6 species was less than 1% of the amount found in the 15 strains. The ability to synthesize lipids from acetate is a useful criterion for differentiating the genus from .

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1974-01-01
2024-12-14
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References

  1. Bak L. B., Black F. T., Christiansen C., Freundt E. A. 1969; Genome size of mycoplasmal DNA. Nature (London) 224:1209–1210
    [Google Scholar]
  2. Bode H. R., Morowitz H. J. 1967; Size and structure of the Mycoplasma hominis H39 chromosome. J. Mol. Biol 23:191–199
    [Google Scholar]
  3. Kunze M. 1971; Natrium-Polyanethol-Sulfonate als diagnostisches Hilfsmittel bei der Differenzierung von Mykoplasmen. Zentralbl. Bakteriol. Parasitenk. Infektionskr 216:501–505
    [Google Scholar]
  4. Leach R. H. 1973; Further studies on classification of bovine strains of Mycoplasmatales, with proposals for new species, Acholeplasma modicum and Mycoplasma alkalescens.. J. Gen. Microbiol 75:135–153
    [Google Scholar]
  5. Mårdh P. -A., Taylor-Robinson D. 1973; The differential effect of lysolecithin on mycoplasmas and acholeplasmas. Med. Microbiol. Immunol 158:219–226
    [Google Scholar]
  6. Metcalfe L. D., Schmitz A. A., Pelka J. R. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Anal. Chem 38:514–515
    [Google Scholar]
  7. Morowitz H. J., Bode H. R., Kirk R. G. The nucleic acids of mycoplasma. Ann. N. Y. Acad. Sci 143:110–114
    [Google Scholar]
  8. Plackett P., Smith P. F., Mayberry W. R. Lipids of a sterol-nonrequiring mycoplasma. J. Bacteriol 104:798–807
    [Google Scholar]
  9. Pollack J. D., Clark D. S., Somerson N. L. Four-directional-development thin-layer chromatography of lipids using trimethyl borate. J. Lipid Res 12:563–569
    [Google Scholar]
  10. Pollack J. D., Razin S., Cleverdon R. C. 1965; Localization of enzymes in Mycoplasma.. J. Bacteriol 90:617–622
    [Google Scholar]
  11. Pollack J. D., Razin S., Pollack M.E., Cleverdon R. C. 1965; Fractionation of mycoplasma cells for enzyme localization. Life Sci 4:973–977
    [Google Scholar]
  12. Pollack J. D., Somerson N. L., Senterfit L. B. 1969; Effect of pH on immunogenicity of M. pneumoniae.. J. Bacteriol 97:612–619
    [Google Scholar]
  13. Pollack J. D., Somerson N. L., Senterfit L. B. 1970; Isolation, characterization immunogenicity of Mycoplasma pneumoniae membranes. Infect. Immunity 2:326–339
    [Google Scholar]
  14. Pollack J. D., Somerson N. L., Senterfit L. B. 1973; Chemical composition and serology of Mycoplasma pneumoniae lipids. J. Infect. Dis 127:32–35
    [Google Scholar]
  15. Pollack J. D., Tourtellotte M. E. 1967; Synthesis of saturated long chain fatty acids from sodium acetate-1-14 >C by Mycoplasma. J. Bacterid 93:636–641
    [Google Scholar]
  16. Prescott B., Caldes G., James W.D., Chanock R. M. 1967; Phospholipid hapten of Mycoplasma pneumoniae.. Ann. N. Y. Acad. Sci 143:497–500
    [Google Scholar]
  17. Razin S., Argaman M., Avignan J. 1963; Chemical composition of mycoplasma cells and membranes. J. Gen. Microbiol 33:477–487
    [Google Scholar]
  18. Razin S., Tully J. G. 1970; Cholesterol requirement of mycoplasmas. J. Bacteriol? 102:306–310
    [Google Scholar]
  19. Robinson J. P., Hungate R. E. 1973; Acholeplasma bactoclasticum sp. n., an anaerobic mycoplasma from the bovine rumen. Int. J. Syst. Bacteriol 23:171–181
    [Google Scholar]
  20. Rodwell A.W. 1967; The nutrition and metabolism of mycoplasma: progress and problems. Ann. N. Y. Acad. Sci 143:88–109
    [Google Scholar]
  21. Rottem S. 1972; Differentiation of sterol-requiring from sterol-nonrequiring mycoplasmas by amphotericin B. Appl. Microbiol 23:659–660
    [Google Scholar]
  22. Rottem S., Muhsam-Peled O., Razin S. 1973; Acyl carrier protein in mycoplasmas. J. Bacteriol 113:586–591
    [Google Scholar]
  23. Rottem S., Razin S. 1967; Uptake and utilization of acetate by mycoplasmas. J. Gen. Microbiol 48:53–63
    [Google Scholar]
  24. Rouser G., Kritchevsky G., Yamomoto A. 1967; Column chromatographic and associated procedures for separation and determination of phosphatides and glycolipids, p.. 99–162 In Marinetti G. V. (ed.) Lipid chromatographic analysis. Marcel Dekker, Inc.; New York:
    [Google Scholar]
  25. Siakotos A. N., Rouser G. 1965; Analytical separation of nonlipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography. J. Amer. Oil Chem. Soc 42:913–919
    [Google Scholar]
  26. Smith P.F. 1968; lipids of Mycoplasma. p. 69–105 In Paoletti R., Kritchevsky D. (ed.) Advances in lipid research Academic Press Inc; New York:
    [Google Scholar]
  27. Smith P.F. 1969; Biosynthesis of glucosyl diglycerides by Mycoplasma laidlawii strain B. J. Bacteriol 99:480–486
    [Google Scholar]
  28. Smith P.F. 1972; Lipid composition of Mycoplasma neurolyticum.. J. Bacteriol 112:554–558
    [Google Scholar]
  29. Smith P. F., Rothblat G. H. 1960; Incorporation of cholesterol by pleuropneumonia-like organisms. J. Bacteriol 80:842–850
    [Google Scholar]
  30. Tully J. G., Razin S. 1969; Characteristics of a new sterol-nonrequiring Mycoplasma.. J. Bacteriol 98:970–978
    [Google Scholar]
  31. Turner J. D., Rouser G. 1970; Precise quantitative determination of human blood lipids by thin-layer and triethylamino ethylcellulose column chromatography. II. Plasma lipids. Anal. Biochem 38:437–445
    [Google Scholar]
  32. Vorbeck M. L., Marinetti G. V. 1965; Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography. J. Lipid Res 6:3–6
    [Google Scholar]
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