1887

Abstract

Thirty-five strains of Pseudomonas cepacia Burkholder, isolated over the last 6 years, were compared with three EO-1 strains, two strains of Stanier et al., and the type strains of and Jonsson, the name of the latter having been given to the EO-1 group. The strains were examined in a range of morphological, biochemical, and carbon substrate utilization tests, and, for selected strains, by electron microscopy, deoxyribonucleic acid base composition, serotyping, and phage typing. From the results of these tests, Jonsson 1970 (EO-1 group) is considered a later subjective synonym of Burkholder 1950 (). The outstanding property of is its nutritional versatility, which is reflected in the large number of carbohydrates from which acid is produced by this organism. A table is given for differentiating from , and

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1972-07-01
2024-10-12
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References

  1. Ballard R. W., Palleroni N. J., Doudoroff M., Stanier R. Y., Mandel M. 1970; Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. J. Gen. Microbiol. 60:199–214
    [Google Scholar]
  2. Bassett D. C. J., Stokes K. J., Thomas W. R. G. 1970; Wound infection with Pseudomonas multivorans. Lancet 1:1188–1191
    [Google Scholar]
  3. Burdon D. W., Whitby J. C. 1967; Contamination of hospital disinfectants with Pseudomonas species. Brit. Med. J. ii:153–155
    [Google Scholar]
  4. Burkholder W. H. 1950; Sour skin, a bacterial rot of onion bulbs. Phytopathology 40:115–117
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the identification of medical bacteria. University Press; Cambridge:
    [Google Scholar]
  6. Crosby N. T. 1967; The determination of nitrite in water using Cleve’s acid, 1-naphthylamine-7- sulphonic acid. J. Soc. Water Treatment Exam. 16:51–55
    [Google Scholar]
  7. Daily R. H., Benner E. J. 1968; Necrotizing pneumonitis due to the pseudomonad “Eugonic Oxidizer-group l”. N. Engl. J. Med. 279:361–362
    [Google Scholar]
  8. Gilardi G. L. 1970; Characterization of EO-1 strains (Pseudomonas kingii) isolated from clinical specimens and the hospital environment. Appl. Microbiol. 20:521–522
    [Google Scholar]
  9. Gilardi G. L. 1971; Characterization of Pseudomonas species isolated from clinical specimens. Appl. Microbiol. 21:414–419
    [Google Scholar]
  10. Gordon R. E., Smith M. M. 1955; Rapidly growing, acid fast bacteria. 11. Species’ description of Mycobacterium fortuitum Cruz. J. Bacteriol. 69:502–507
    [Google Scholar]
  11. Graber C. D., Jervey L. P., Ostrander W. E., Salley L. H., Weaver R. E. 1968; Endocarditis due to a lanthanic, unclassified gram-negative bacterium (group IVd). Amer. J. Clin. Pathol. 49:220–223
    [Google Scholar]
  12. Hardy P. C., Ederer G. M., Matsen J. M. 1970; Contamination of commercially packed urinary catheter kits with the pseudomonad EO-1. N. Engl. J. Med. 282:33–35
    [Google Scholar]
  13. Hill L. R., Leach R. H., Andrews B. E. 1970; The value of DNA base composition determinations in the classification of some atypical mycoplasmas. Spisy Prirodoved. Fak. Univ. Brne Ser. K 47:79–83
    [Google Scholar]
  14. Hill L. R., Snell J. J. S., Lapage S. P. 1970; Identification and characterization of Bacteroides corrodens. J. Med. Microbiol. 3:483–491
    [Google Scholar]
  15. Hugh R. 1970; A practical approach to the identification of certain nonfermentative gramnegative rods encountered in clinical specimens. Pub. Health Lab. 28:168–187
    [Google Scholar]
  16. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J. Bacteriol. 66:24–26
    [Google Scholar]
  17. Jonsson V. 1970; Proposal of a new species Pseudomonas kingii. Int. J. Syst. Bacteriol. 20:255–257
    [Google Scholar]
  18. King E. O. 1964 The identification of unusual pathogenic Gram negative bacteria. National Communicable Disease Center; Atlanta:
    [Google Scholar]
  19. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescin. J. Lab. Clin. Med. 44:301–307
    [Google Scholar]
  20. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature (London) 178:703
    [Google Scholar]
  21. Lapage S. P., Bascomb S. 1968; Use of selenite reduction in bacterial classification. J. Appl. Bacteriol. 31:568–580
    [Google Scholar]
  22. Lapage S. P., Hill L. R., Reeve J. D. 1968; Pseudomonas stutzeri in pathological material. J. Med. Microbiol. 1:195–202
    [Google Scholar]
  23. Mackel D. C. 1970; Contamination of disposable catheter kits with EO-1. N. Engl. J. Med. 282:752–753
    [Google Scholar]
  24. Mandel M. 1966; Deoxyribonucleic acid base composition in the genus Pseudomonas. J. Gen. Microbiol. 43:273–292
    [Google Scholar]
  25. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3:208–218
    [Google Scholar]
  26. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5:109–118
    [Google Scholar]
  27. Mitchell R. G., Hayward A. C. 1966; Postoperative urinary-tract infections caused by contaminated irrigating fluid. Lancet 1:793–795
    [Google Scholar]
  28. Müller V. 1955; Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system. Acta Pathol. Microbiol. Scand. 36:158–172
    [Google Scholar]
  29. Morris M. B., Roberts J. B. 1959; A group of pseudomonads able to synthesize poly-β-hydroxybutyric acid. Nature (London) 183:1538–1539
    [Google Scholar]
  30. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers 7:503–516
    [Google Scholar]
  31. Owens J. D., Keddie R. M. 1968; A note on the vitamin requirements of some coryneform bacteria from soil and herbage. J. Appl. Bacteriol. 31:344–348
    [Google Scholar]
  32. Phillips I., Eykyn S., Curtis M. A., Snell J. J. S. 1971; Pseudomonas cepacia (multivorans) septicaemia in an intensive-care unit. Lancet 1:375–377
    [Google Scholar]
  33. Pickett M. J., Pedersen M. M. 1970; Characterization of saccharolytic nonfermentative bacteria associated with man. Can J. Microbiol. 16:351–362
    [Google Scholar]
  34. Plimmer H. G., Paine S. G. 1921; A new method for the staining of bacterial flagella. J. Pathol. Bacteriol. 24:286–288
    [Google Scholar]
  35. Redfearn M. S., Palleroni N. J., Stanier R. Y. 1966; A comparative study of Pseudomonas pseudomallei and Bacillus mallei. J. Gen. Microbiol. 43:293–313
    [Google Scholar]
  36. Richard C. 1968; Techniques rapides de recherche des lysine-decarboxylase, ornithinedecarboxylase et arginine-dihydrolase dans les genres Pseudomonas, Alcaligenes et Moraxella. Ann. Inst. Pasteur 114:425–430
    [Google Scholar]
  37. Sands D. C., Schroth M. N., Hildebrand D. C. 1970; Taxonomy of phytopathogenic pseudomonads. J. Bacteriol. 101:9–23
    [Google Scholar]
  38. Sierra G. 1957; A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek J. Microbiol. Serol. 23:15–22
    [Google Scholar]
  39. Smith N. R., Gordon R. E., Clark F. E. 1952 Aerobic spore forming bacteria. Agriculture Monograph No. 16 U.S. Department of Agriculture;
    [Google Scholar]
  40. Sneath P. H. A. 1956; Cultural and biochemical characteristics of the genus Chromobacterium. J. Gen. Microbiol. 15:70–98
    [Google Scholar]
  41. Sneath P. H. A. 1960; A study of the bacterial genus Chromobacterium. Iowa State Coll. J. Sci. 34:243–500
    [Google Scholar]
  42. Speller D. C. E., Stephens M. E., Viant A. C. 1971; Hospital infection by Pseudomonas cepacia. Lancet 1:798–799
    [Google Scholar]
  43. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. J. Gen Microbiol. 43:159–271
    [Google Scholar]
  44. Taplin D., Bassett D. C. J., Mertz P. M. 1971; Foot lesions associated with Pseudomonas cepacia. Lancet 2:568–571
    [Google Scholar]
  45. Thornley M. J. 1960; The differentiation of Pseudomonas from other Gram negative bacteria on the basis of arginine metabolism. J. Appl. Bacteriol. 23:37–52
    [Google Scholar]
  46. Tweedy J. M., Park R. W. A., Hodgkiss W. 1968; Evidence for the presence of fimbriae (pili) on vibrio species. J. Gen. Microbiol. 51:235–244
    [Google Scholar]
  47. Véron M. 1961; Sur l’agglutination de Pseudomonas aeruginosa 0:2 et 0:5. Ann. Inst. Pasteur 101:456–460
    [Google Scholar]
  48. Yabuuchi E., Miyajima N., Hotta H., Ohyama A., Tanaka N. 1970; Pseudomonas cepacia from blood of a burn patient. Med. J. Osaka Univ. 21:1–6
    [Google Scholar]
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