1887

Abstract

SET domain genes have been identified in numbers of bacterial genomes based on similarity to SET domains of eukaryotic histone methyltransferases. Herein, a SET domain gene was clarified to be coincidently expressed with and genes encoding chlamydial histone H1-like proteins, Hc1 and Hc2, respectively. The SET domain protein (cpnSET) is localized in chlamydial cells and interacts with Hc1 and Hc2 through the C-terminal SET domain. As expected from conservation of catalytic sites in cpnSET, it functions as a protein methyltransferase to murine histone H3 and Hc1. However, little is known about protein methylation in the molecular pathogenesis of chlamydial infection. cpnSET may play an important role in chlamydial cell maturation due to modification of chlamydial histone H1-like proteins.

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2007-02-01
2019-11-13
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Supplements

Primer sequences for vector construction and quantitative PCR [ PDF] (20 kb) Structure-based sequence alignment of SET domains [ PDF] (200 kb) Interaction analysis between cpnSET and Hc1, and cpnSET and Hc2 [ PDF] (375 kb) Informatics analysis of methylation site(s) of Hc1 by cpnSET [ PDF] (66 kb)

PDF

Primer sequences for vector construction and quantitative PCR [ PDF] (20 kb) Structure-based sequence alignment of SET domains [ PDF] (200 kb) Interaction analysis between cpnSET and Hc1, and cpnSET and Hc2 [ PDF] (375 kb) Informatics analysis of methylation site(s) of Hc1 by cpnSET [ PDF] (66 kb)

PDF

Primer sequences for vector construction and quantitative PCR [ PDF] (20 kb) Structure-based sequence alignment of SET domains [ PDF] (200 kb) Interaction analysis between cpnSET and Hc1, and cpnSET and Hc2 [ PDF] (375 kb) Informatics analysis of methylation site(s) of Hc1 by cpnSET [ PDF] (66 kb)

PDF

Primer sequences for vector construction and quantitative PCR [ PDF] (20 kb) Structure-based sequence alignment of SET domains [ PDF] (200 kb) Interaction analysis between cpnSET and Hc1, and cpnSET and Hc2 [ PDF] (375 kb) Informatics analysis of methylation site(s) of Hc1 by cpnSET [ PDF] (66 kb)

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