1887

Abstract

is a common wood-rotting fungus parasitic for birch ( species). It is able to cause fast mass loss of birch wood or other lignocellulose substrates. When grown on wheat straw, caused 65 % loss of dry mass within 98 days, and it produced endo-1,4--glucanase (EG), endo-1,4--xylanase, endo-1,4--mannanase, 1,4--glucosidase (BG), 1,4--xylosidase, 1,4--mannosidase and cellobiohydrolase activities. The fungus was not able to efficiently degrade crystalline cellulose. The major glycosyl hydrolases, endoglucanase EG1 and -glucosidase BG1, were purified. EG1 was a protein of 62 kDa with a pI of 2.6–2.8. It cleaved cellulose internally, produced cellobiose and glucose from cellulose and cellooligosaccharides, and also showed -xylosidase and endoxylanase activities. The for carboxymethylcellulose was 3.5 g l, with the highest activity at pH 3.5 and 70 °C. BG1 was a protein of 36 kDa with a pI around 2.6. It was able to produce glucose from cellobiose and cellooligosaccharides, but also produced galactose, mannose and xylose from the respective oligosaccharides and showed some cellobiohydrolase activity. The for -nitrophenyl-1,4--glucoside was 1.8 mM, with the highest activity at pH 4 and 60 °C, and the enzyme was competitively inhibited by glucose ( =5.8 mM). The fungus produced mainly -glucosidase and -mannosidase activity in its fruit bodies, while higher activities of endoglucanase, endoxylanase and -xylosidase were found in fungus-colonized wood.

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2006-12-01
2024-12-09
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