1887

Abstract

A highly conserved cryptic plasmid is present in yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of , using novobiocin as a curing agent. Plasmid-deficient derivatives of strain Nigg were generated at high efficiencies (4–30 %). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.

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2006-06-01
2020-09-21
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