@article{mbs:/content/journal/micro/10.1099/mic.0.28658-0, author = "O'Connell, Catherine M. and Nicks, Kristy M.", title = "A plasmid-cured Chlamydia muridarum strain displays altered plaque morphology and reduced infectivity in cell culture", journal= "Microbiology", year = "2006", volume = "152", number = "6", pages = "1601-1607", doi = "https://doi.org/10.1099/mic.0.28658-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.28658-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "i.f.u., inclusion-forming units", abstract = "A highly conserved cryptic plasmid is present in Chlamydia trachomatis yet naturally occurring plasmid-deficient isolates are very rare. This paper describes the isolation and characterization of a plasmid-deficient strain of C. muridarum, using novobiocin as a curing agent. Plasmid-deficient derivatives of C. muridarum strain Nigg were generated at high efficiencies (4–30 %). Phenotypic characterization revealed that the cured derivative was unable to accumulate glycogen within intracytoplasmic inclusions. In addition, this strain formed small plaques at a reduced efficiency compared to the wild-type parent.", }