1887

Abstract

The long-chain acyl-CoA synthase (ACS) FadD1 plays an important role in timing the levels of antibiotic production in and , encoding a putative medium-chain ACS, are part of a two-gene operon, whose expression is induced during the stationary phase of growth. Here it is reported that transcription of the operon is positively regulated by AcsR, a LuxR-type transcriptional regulator. In an mutant, expression of the genes loses its normal up-regulation and the mutant becomes deficient in antibiotic production, in a clear correlation with the phenotype shown by a null mutant. The absence of induction in the mutant was restored by complementation with a wild-type copy of the gene, showing a strict link between AcsR and induction of the operon. Gel mobility shift assays and DNase I footprinting indicated that AcsR binds to specific sequences about +162 nucleotides downstream of the transcriptional start site. In the putative operator sequence three almost identical direct tandem repeats of seven nucleotides were identified where the central sequence is essential for AcsR recognition and binding. Transcriptional fusions of the divergent p and p promoters indicated that AcsR does not regulate its own transcription, and that it binds to the operator region to control exclusively the growth-phase induction of the operon.

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2006-05-01
2019-10-18
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