1887

Abstract

is a strictly anaerobic methanogenic archaeon, which can survive oxidative stress. The oxidative stress agent paraquat (PQ) suppressed growth of at concentrations of 50–200 μM. Hydrogen peroxide (HO) inhibited growth at concentrations of 0.4–1.6 mM. Catalase activity in cell-free extracts of increased about threefold during HO stress (1.3 mM HO, 2–4 h exposure) and nearly twofold during superoxide stress (160 μM PQ, 2 h exposure). PQ (160 μM, 2–4 h exposure) and HO (1.3 mM, 2 h exposure) also influenced superoxide dismutase activity in cell-free extracts of . Dot-blot analysis was performed on total RNA isolated from HO- and PQ-exposed cultures, using labelled internal DNA fragments of the and genes. It was shown that HO but not PQ strongly induced up-regulation of the gene. PQ and to a lesser degree HO induced the expression of superoxide dismutase. The results indicate the regulation of the adaptive response of to different oxidative stresses.

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2006-06-01
2020-07-13
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