1887

Abstract

The phylogenically related and species are two ruminant pathogens difficult to differentiate and for which a limited amount of sequence data are available. To assess the degree of genomic diversity existing between and within these mycoplasma species, sets of DNA fragments specific for type-strain PG45 or for type-strain PG2 were isolated by suppression subtractive hybridization and used as probes on a panel of and field isolates. Results indicated that approximately 70 % of the DNA fragments specific to one or the other type strain are represented in all field isolates of the corresponding species. Only one isolate, which was first classified as , reacted with 15 % of the PG2-specific probes, while several isolates reacted with 15 % of the PG45-specific probes. Sequence analyses indicated that most of the genomic diversity observed within one species is related to ORFs with (i) no homologies to proteins recorded in the databases or (ii) homologies to proteins encoded by restriction modification systems. Reminiscent of gene transfer as a means for genomic diversity, a PG45-specific DNA fragment with significant homologies to a central protein of an integrative conjugative element of (ICEF) was found in most field isolates and in a few isolates. Finally, sequences encoding part of DNA polymerase III were found in both sets of - and -specific DNA fragments and were used to design a species-specific PCR assay for the identification and differentiation of and .

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2005-02-01
2020-04-04
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