1887

Abstract

Space flight has been shown to affect various bacterial growth parameters. It is proposed that weightlessness allows the cells to remain evenly distributed, consequently altering the chemical makeup of their surrounding fluid, and hence indirectly affecting their physiological behaviour. In support of this argument, ground-based studies using clinostats to partially simulate the quiescent environment attained in microgravity have generally been successful in producing bacterial growth characteristics that mimic responses reported under actual space conditions. A novel approach for evaluating the effects of reduced cell sedimentation is presented here through use of cultures genetically modified to be neutrally buoyant. Since clinorotation would not (or would only minimally) affect cell distribution of this already near-colloidal cell system, it was hypothesized that the effects on final population density would be eliminated relative to a static control. Gas-vesicle-producing cultures were grown under clinostat and static conditions and the culture densities at 60 h were compared. As a control, that do not produce gas vesicles, but were otherwise identical to the experimental strain, were also grown under clinostat and static conditions. As hypothesized, no significant difference was observed in cell populations at 60 h between the clinorotated and static gas-vesicle-producing cultures, while the cells that did not produce gas vesicles showed a mean increase in population density of 10·5 % (=0·001). These results further suggest that the lack of cumulative cell sedimentation is the dominant effect of space flight on non-stirred, cultures.

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2005-01-01
2019-11-21
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vol. , part 1, pp. 69 - 74

Details of the calculation for percentage of cells sedimented in a horizontally oriented fluid processing apparatus (FPA) are given in the accompanying PDF file.



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