1887

Abstract

The regulation of the expression of the operon, proposed to encode an electron transfer chain from the outer to the inner membrane in the obligate acidophilic chemolithoautroph , has been studied at the RNA and protein levels. As observed by Northern hybridization, real-time PCR and reverse transcription analyses, this operon was more highly expressed in ferrous iron- than in sulfur-grown cells. Furthermore, it was shown by immunodetection that components of this respiratory chain are synthesized in ferrous iron- rather than in sulfur-growth conditions. Nonetheless, weak transcription and translation products of the operon were detected in sulfur-grown cells at the early exponential phase. The results strongly support the notion that -operon expression is induced by ferrous iron, in agreement with the involvement of the -operon-encoded products in the oxidation of ferrous iron, and that ferrous iron is used in preference to sulfur.

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2004-07-01
2019-11-22
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