1887

Abstract

The response regulator AgmR was identified to be involved in the regulation of the quinoprotein ethanol oxidation system of ATCC 17933. Interruption of the gene by insertion of a kanamycin-resistance cassette resulted in mutant NG3, unable to grow on ethanol. After complementation with the intact gene, growth on ethanol was restored. Transcriptional fusions were used to identify four operons which are regulated by the AgmR protein: the operon encodes the pyrroloquinoline quinone (PQQ)-dependent ethanol dehydrogenase, the operon encodes a soluble cytochrome and an aldehyde dehydrogenase, the operon carries the PQQ biosynthetic genes, and operon encodes a two-component regulatory system which controls transcription of the operon. Transcription of was restored by transformation of NG3 with a pUCP20T derivative carrying the genes under -promoter control. These data indicate that the AgmR response regulator and the two-component regulatory system are organized in a hierarchical manner. Gene PA1977, which appears to form an operon with the gene, was found to be non-essential for growth on ethanol.

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2004-06-01
2020-03-29
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