1887

Abstract

The expression of genes for cold-shock proteins is proposed to be regulated primarily at the post-transcriptional level by increase of mRNA stability after transition to low temperatures. Destabilization of the cold-induced transcript at 37 °C as well as stabilization upon cold shock is known to depend on the unusually long (159 nt) 5′-untranslated region. Determination of the mRNA 5′-end from revealed a shorter distance between the start of transcription and the start codon for translation. The mRNA of was shown to be stabilized at low temperatures to a greater extent than other investigated transcripts. To address the mechanism of decay of the transcript, it was incubated with purified degradosome of . Endoribonucleolytic cleavage in the 5′-untranslated region as reported for the transcript of was not observed. Instead, the data indicated that the mRNA decay in is mediated by endoribonucleolytic cleavages within the coding region.

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2004-03-01
2019-11-21
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