1887

Abstract

Natural transformation, a mechanism that generates genetic diversity in , was studied in a novel liquid shake culturing system that allowed an approximately 10 000-fold increase in cell density. transformation frequency was analysed in this system under 10 %, 5·0 % and 0·7 % CO atmospheres. At 5·0 % and 10 % CO concentrations, when purified isogenic chromosomal DNA was used to assess competence, transformation frequency ranged from 10 to 10 at low cell concentrations and declined as cell density increased. Transformation frequency under a 0·7 % CO atmosphere was more stable, maintaining 10 levels at high cell densities, and was 10- to 100-fold higher than that under a 10 % CO atmosphere. Three of four strains tested under a 5·0 % CO atmosphere were naturally competent for isogenic DNA; competent strains demonstrated a lack of barriers to intraspecies genetic exchange by taking up and incorporating chromosomal DNA from multiple donors. showed a preference for its own DNA at the species level, and co-cultivation demonstrated that DNA transfer via natural transformation occurred between isogenic populations during short periods of exposure in liquid medium when cell density and presumably DNA concentrations were low. Transformation frequency during co-cultivation of isogenic populations was also influenced by CO concentration. Under a 0·7 % CO atmosphere, co-cultivation transformation frequency increased approximately 500-fold in a linear fashion with regard to cell density, and was 1000- to 10 000-fold higher during late-exponential-phase growth when compared to cultures grown under a 10 % CO atmosphere.

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2003-12-01
2019-11-21
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