1887

Abstract

The glycopeptide teicoplanin is used for the treatment of serious infections caused by Gram-positive pathogens. The gene cluster, devoted to teicoplanin biosynthesis in the actinomycete , was isolated and characterized. From sequence analysis, the cluster spans approximately 73 kb and includes 39 ORFs participating in teicoplanin biosynthesis, regulation, resistance and export. Of these, 34 ORFs find a match in at least one of the five glycopeptide gene clusters previously characterized. Putative roles could be assigned for most of the genes. The two glycosyltransferases responsible for attaching amino sugars to amino acids 4 and 6 of the teicoplanin aglycon were overexpressed in and characterized. They both recognize -acetylglucosamine as the substrate. tGtfA can add a sugar residue in the presence or absence of -acetylglucosamine at amino acid 4, while tGtfB can only glycosylate the teicoplanin aglycon.

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2004-01-01
2019-11-22
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