RT Journal Article SR Electronic(1) A1 Sosio, Margherita A1 Kloosterman, Harm A1 Bianchi, Alessandra A1 de Vreugd, Peter A1 Dijkhuizen, Lubbert A1 Donadio, StefanoYR 2004 T1 Organization of the teicoplanin gene cluster in Actinoplanes teichomyceticus JF Microbiology, VO 150 IS 1 SP 95 OP 102 DO https://doi.org/10.1099/mic.0.26507-0 PB Microbiology Society, SN 1465-2080, AB The glycopeptide teicoplanin is used for the treatment of serious infections caused by Gram-positive pathogens. The tcp gene cluster, devoted to teicoplanin biosynthesis in the actinomycete Actinoplanes teichomyceticus, was isolated and characterized. From sequence analysis, the tcp cluster spans approximately 73 kb and includes 39 ORFs participating in teicoplanin biosynthesis, regulation, resistance and export. Of these, 34 ORFs find a match in at least one of the five glycopeptide gene clusters previously characterized. Putative roles could be assigned for most of the tcp genes. The two glycosyltransferases responsible for attaching amino sugars to amino acids 4 and 6 of the teicoplanin aglycon were overexpressed in Escherichia coli and characterized. They both recognize N-acetylglucosamine as the substrate. tGtfA can add a sugar residue in the presence or absence of N-acetylglucosamine at amino acid 4, while tGtfB can only glycosylate the teicoplanin aglycon., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26507-0